Sel-Plex® Improves Spermatozoa Morphology in Broiler Breeder Males
DOI:
https://doi.org/10.3923/ijps.2009.853.861Keywords:
Broiler breeder, Sel-Plex®, selenium, semen, spermatozoa, testesAbstract
Sodium Selenite (SEL) has been the traditional source of Selenium (Se) in poultry diets, but Sel-Plex® (SP, a source of organic selenium in yeast protein, Alltech Inc.) has become widely used in several countries signaling its importance as a replacement for SEL. SP is equivalent or even superior to SEL in terms of gut absorption, performance, induction of whole body feathering and tissue retention. Therefore, it was important to extend our understanding of the influence of selenium on performance characteristics of poultry by comparing the influence of SEL or SP in broiler breeder roosters. In the first part of this investigation, 14-week-old roosters were fed diets that contained SEL, SP, or no supplemental selenium (deficient). Selenium-supplemented roosters produced semen at 19 weeks of age while selenium-deficient roosters did not produce semen until 26 weeks of age. Semen quality, as indicated by spermatozoal morphology, was best for SP-fed roosters and SEL-fed roosters produced semen with a quality that was intermediate between SP-fed and selenium-deficient rooster semen quality. In the second part of this investigation, adult roosters in a commercial setting were fed SEL at 0.3 ppm Se/kg of diet until they were 19 weeks of age and then half of the males on each of two farms were fed SP at 0.3 ppm Se/kg of diet. At 32 and 42 weeks of age, semen samples were evaluated via microscopy for quality based on spermatozoal morphology and spectrophotometric analysis to determine a sperm quality index, consisting of a composite determined by sperm motility and sperm density in the semen sample. The results from the laboratory trial and the field trial suggest that SP is superior to SEL as a source of selenium for broiler breeder males. This conclusion was further supported by histological evaluation of testicular tissues from roosters fed either no supplemental selenium, SP or SEL.
References
Allan, C.B., G.M. Lacourciere and T.C. Stadtman, 1999. Responsiveness of selenoproteins to dietary selenium. Annu. Rev. Nutr., 19: 1-16.
Behne, D., T. Hofer, R. von Berswordt-Wallrabe and W. Elger, 1982. Selenium in the testis of the rat: Studies on its regulation and its importance for the organism. J. Nutr., 112: 1682-1687.
Behne, D., M. Duh and W. Elger, 1986. Selenium content and glutathione peroxidase activity in the testis of the maturing rat. J. Nutr., 116: 1442-1447.
Behne, D., H. Hilmert, S. Scheid, H. Gessner and W. Elger, 1988. Evidence for specific selenocysteine target tissues and new biologically important selenoproteins. Biochem. Biophys. Acta, 966: 12-21.
Behne, D., T. Hofer-Bosse and W. Elger, 1987. Selenium and Hormones in the Male Reproductive System. In: Selenium in Biology and Medicine, Combs, Jr. G.F., J.E. Spallholz, O.A. Levander and J.E. Oldfield (Eds.). Van Nostrand Reinhold Co., New York, USA., pp: 733-739.
Blom, E., 1950. A one-minute live-dead sperm stain by means of eosin-nigrosin. Fertil. Steril., 1: 176-177.
Burk, R.J., 1978. Selenium in nutrition. World Rev. Nutr. Diet, 30: 88-106.
Burrows, W.H. and J.P. Quinn, 1935. A method of obtaining spermatozoa from the domestic fowl. Poult. Sci., 14: 251-254.
Burrows, W.H. and J.P. Quinn, 1937. The collection of spermatozoa from the domestic fowl and Turkey. Poult. Sci., 16: 19-24.
Combs, Jr. G.F. and S.B. Combs, 1986. The Role of Selenium in Nutrition. Academic Press, Boca Raton, Florida.
Csallany, A.S. and B.Z. Menken, 1986. Effect of dietary selenite on hepatic organic solvent-soluble lipofuscin pigments. J. Am. Coll. Toxicol., 5: 79-85.
Edens, F.W., 1970. Reproductive characteristics in lines of chickens selected for high and low juvenile body weight. M.Sc. Thesis, The Graduate School, Virginia Polytechnic Institute and State University, Blacksburg, Virginia.
Froman, D.P., A.J. Feltmann, M.L. Rhoads and J.D. Kirby, 1999. Sperm mobility: A primary determinant of fertility in the domestic fowl (Gallus domesticus). Biol. Reprod., 61: 400-405.
Gonzalez-Moran, M.G., C. Guerra-Araiza, M.G. Campos and I. Camacho-Arroyo, 2008. Histological and sex steroid hormone receptor changes in testes of immature, mature and aged chickens. Domestic Anim. Endocrinol., 35: 371-379.
Hafeman, D.G., R.A. Sunde and W.G. Hoekstra, 1974. Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. J. Nutr., 104: 580-587.
Hansen, J.C. and Y. Deguchi, 1996. Selenium and fertility in animals and man: A review. Acta Vet. Scand., 37: 19-30.
Kleene, K.C., 1994. The Mitochondrial Capsule Selenoprotein-A Structural Protein in the Mitochondrial Capsule of Mammalian Sperm. In: Selenium in Biology and Human Medicine, Burk, R.F. (Ed.). Springer Verlag, New York, pp: 135-148.
Ostle, B., 1963. Statistics in Research B. Iowa State University Press, Ames, IA., USA.
Parker, H.M., A.G. Karaca, J.B. Yeatman, L.R. Frank and C.D. McDaniel, 2002. Fertility of broiler breeders following categorization by the optibreed sperm quality index when hens are inseminated with a constant number of sperm. Poult. Sci., 81: 239-245.
Rosenstrauch, A., A.A. Degen and M. Friedlander, 1994. Spermatozoa retention by Sertoli cells during decline in fertility in aging roosters. Biol. Reprod., 50: 129-136.
SAS Institute, 1996. A User's Guide to the Statistical Analysis System. SAS Institute, Cary, NC., USA.
Sikka, S.C., 1996. Oxidative stress and role of antioxidants in normal and abnormal sperm function. Frontiers Biosci., 1: 78-86.
Spallholz, J.E., 1997. Free radical generation by selenium compounds and their pro-oxidant toxicity. Biomed. Environ. Sci., 10: 260-270.
Sprinker, L.H., J.R. Harr, P.M. Newberne, P.D. Whanger and P.H. Weswig, 1971. Selenium deficiency lesions in rats fed vitamin E-supplemented rations. Nutr. Rep. Int., 4: 335-340.
Surai, P.F., 2000. Organic selenium: Benefits to animals and humans, a biochemists view. Proceedings of the Alltech's 16th Annual Symposium, (AAS'00), Thrumpton, Nottingham, pp: 205-260.
Surai, P.F., E. Blesbois, I. Grasseau, T. Chalah and J.P. Brillard et al., 1998. Fatty acid composition, glutathione peroxidase and superoxide dismutase activity and total antioxidant activity of avian semen. Comp. Biochem. Physiol. B Biochem. Mol. Biol., 120: 527-533.
Surai, P., I. Kostjuk, G. Wishart, A. MacPherson and B. Speake et al., 1998. Effect of vitamin E and selenium supplementation of cockerel diets on glutathione peroxidase activity and lipid peroxidation susceptibility in sperm, testes and liver. Biol. Trace Elem. Res., 64: 119-132.
Surai, P.F., N. Fujihara, B.K. Speake, J.P. Brillard, G.J. Wishart and N.H.C. Sparks, 2001. Polyunsaturated fatty acids, lipid peroxidation and antioxidant protection in avian semen. Asian-Australas. J. Anim. Sci., 14: 1024-1050.
Terada, A., M. Yoshida, Y. Seko, T. Kobayashi and K. Yoshida et al., 1999. Active oxygen species generation and cellular damage by additives of parenteral preparations: Selenium and sulfhydryl compounds. Nutretion, 15: 651-655.
Ursini, F., S. Heim, M. Kliess, M. Maiorino, A. Roveri, J. Wissing and L. Flohe, 1999. Dual function of the selenoprotein PHGPx during sperm maturation. Science, 285: 1393-1396.
Vohra, P., C.M. Johnson, L.Z. McFarland, T.D. Siopes, W.O. Wilson and C.M. Winget, 1973. Role of selenium in neural physiology of avian species. 2. The distribution of 75Se from injected selenomethionine-75Se in the testes and its fate in livers of chickens. Poult. Sci., 52: 644-647.
Wu, A.S., J.E. Oldfield, L.R. Shull and P.R. Cheeke, 1979. Specific effect of selenium deficiency on rat sperm. Biol. Reprod., 20: 793-798.
Sies, H., 1993. Strategies of antioxidant defence. Eur. J. Biochem., 215: 213-219.
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