Immune Responses to Eimeria tenella Sporozoite Protein as Vaccine to Broiler Against Coccidiosis
DOI:
https://doi.org/10.3923/ijps.2013.582.589Keywords:
Eimeria tenella, IL-4, IFN-Y, sporozoite, vaccine, western blotAbstract
The chicken was protected against coccidiosis induce by Eimeria tenella by using sporozoite protein that was injected in the neck subcutaneously with two doses (25 μg per chicken) at 3rd and 16th days of age, vaccinated birds were challenged at 30 days of age. The type of immune response to this vaccine was estimated during vaccination and after the challenge. Blood samples were collected at (7, 28 and 39th) day of age. The immunogenicity of vaccine was studied by using SDS-PAGE and Western blot. 11 polypeptides had been estimated more immunogenic after probing with immunized chicken serum at 39th days of age, their molecular weight are (149.5, 97, 72.9,67.8, 63,51, 38, 17, 13, 10.5 and 8) KD. Also, the levels of γ-IFN and IL-4 were estimated in the serum of immunized chickens by use ELISA kits. The results were demonstrated two types of immunity cellular and humoral responses against E. tenella sporozoite vaccine.
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