Bacteria Recovered from Whole-Carcass Rinsates of Broiler Carcasses Washed in a Spray Cabinet with Lauric Acid-Potassium Hydroxide
DOI:
https://doi.org/10.3923/ijps.2009.1022.1027Keywords:
Broilers, carcasses, lauric acid, potassium hydroxide, spray washingAbstract
Effects of spray washing carcasses with Lauric Acid (LA)-Potassium Hydroxide (KOH) on bacteria recovered from Whole-Carcass Rinsates (WCR) were examined. Carcass skin was inoculated with antibiotic resistant strains of Escherichia coli, Salmonella Typhimirum and Campylobacter coli. The first trial examined effects of washing carcasses with water, 0.25% LA-0.125% KOH, 0.50% LA-0.25% KOH, 1.00% LA-0.50% KOH, or 2.00% LA-1.00% KOH at 80 psi for 15 sec. Findings indicated that significantly fewer Total Plate Count (TPC) bacteria, E. coli and Salmonella Typhimirum were recovered from carcasses washed with 2.00% LA-1.00% KOH than from carcasses washed with water and that no C. coli were recovered from carcasses washed with 2.00% LA-1.00% KOH. Another trial examined effects of washing carcasses at 60, 100, or 150 psi of pressure with 2.00% LA-1.00% KOH for 15 sec. Findings indicated that significantly fewer TPC bacteria were recovered from rinsates of carcasses washed with 100 psi than from carcasses washed with 60 or 150 psi. Finally, a trial was conducted to examine effects of washing carcasses for 0, 5, 15, or 30 sec with 2.00% LA-1.00% KOH at 100 psi. Results indicated that significantly fewer bacteria were recovered from carcasses washed for 5 sec than from unwashed carcasses. Furthermore, significantly fewer TPC bacteria and Salmonella Typhimirum were recovered from carcasses washed for 15 sec than for 5 sec and no C. coli were recovered from carcasses washed for 15 or 30 sec. Findings indicate that spray washing carcasses with LA-KOH can affect the number of bacteria recovered from WCR. These studies also provide data that may be useful in designing applications for using of microbicidal surfactants in processing operations.
References
Bashor, M.P., P.A. Curtis, K.M. Keener, B.W. Sheldon, S. Kathariou and J.A. Osborne, 2004. Effects of carcass washers on Campylobacter contamination in large broiler processing plants. Poult. Sci., 83: 1232-1239.
Berrang, M.E. and J.S. Bailey, 2009. On-line brush and spray washers to lower numbers of Campylobacter and Escherichia coli and presence of Salmonella on broiler carcasses during processing. J. Applied Poult. Res., 18: 74-78.
Blankenship, L.C., N.A. Cox, S.E. Craven, A.J. Mercuri and R.L. Wilson, 1975. Comparison of the microbiological quality of inspection-passed and fecal contamination-condemned broiler carcasses. J. Food Sci., 40: 1236-1238.
Dickens, J.A., N.A. Cox, J.S. Bailey and J.E. Thomson, 1985. Automated microbiological sampling of broiler carcasses. Poult. Sci., 64: 1116-1120.
Galbraith, H. and T.B. Miller, 1973. Physiocochemical effects of long chain fatty acids on bacterial cells and their protoplasts. J. Applied Bacteriol., 36: 647-658.
Hinton, Jr., A. and K.D. Ingram, 2003. Bactericidal activity of tripotassium phosphate and potassium oleate on the native flora of poultry skin. Food Microbiol., 20: 405-410.
Hinton Jr., A. and K.D. Ingram, 2006. Antimicrobial activity of potassium hydroxide and lauric acid towards microorganisms associated with poultry processing. Food Prot., 69: 1611-1615.
Hinton, Jr., A., J.K. Northcutt, J.A. Cason, D.P. Smith and K.D. Ingram, 2007. Bacterial populations of broiler carcasses washed in mixtures of potassium hydroxide and lauric acid. J. Applied Poult. Res., 16: 387-391.
Huang, S.C. and P.Y. Chang, 1997. Optimization of spray-washing conditions for broiler carcasses. China Soc. Anim. Husb., 26: 471-481.
Kabara, J.J., R. Vrable and M.S.F.L.K. Jie, 1977. Antimicrobial lipids: Natural and synthetic fatty acids and monoglycerides. Lipids, 12: 753-759.
Kabara, J.J., 1979. Toxicological, bacteriocidal and fungicidal properties of fatty acids and some derivatives. J. Am. Oil Chem. Soc., 56: 760-767.
Keener, K.M., M.P. Bashor, P.A. Curtis, B.W. Sheldon and S. Kathariou, 2004. Comprehensive review of Campylobacter and poultry processing. Comp. Rev. Food Sci. Food Saf., 3: 105-116.
Li, Y., M.F. Slavik, J.T. Walker and H. Xiong, 1997. Pre-chill spray of chicken carcasses to reduce Salmonella typhimurium. J. Food Sci., 62: 605-607.
Li, Y., H. Yang and B.L. Swem, 2002. Effect of high-temperature inside-outside spray on survival of Campylobacter jejuni attached to prechill chicken carcasses. Poult. Sci., 81: 1371-1377.
Lillard, H.S., 1979. Levels of chlorine and chlorine dioxide of equivalent bactericidal effect in poultry processing water. J. Food Sci., 44: 1594-1597.
Lillard, H.S., 1994. Effect of trisodium phosphate on salmonellae attached to chicken skin. J. Food Prot., 57: 465-469.
Northcutt, J.K., M.E. Berrang, D.P. Smith and D.R. Jones, 2003. Effect of commercial bird washers on broiler carcass microbiological characteristics. J. Applied Poult. Res., 12: 435-438.
Northcutt, J.K., D.P. Smith, M.T. Musgrove, K.D. Ingram and A. Hinton, Jr., 2005. Microbiological impact of spray washing broiler carcasses using different chlorine concentrations and water temperatures. Poult. Sci., 84: 1648-1652.
Russell, S.M., 2007. Chlorine: Still the most popular sanitizer in the poultry industry. http://www.thepoultrysite.com/articles/1383/chlorine-still-the-most-popular-sanitizer-in-the-poultry-industry.
Sakhare, P.Z., N.M. Sachindra, K.P. Yashoda and D.N. Rao, 1999. Efficacy of intermittent decontamination treatments during processing in reducing the microbial load on broiler chicken carcass. Food Control, 10: 189-194.
Zhao, C., B. Ge, J.D. Villena, R. Sudler and E. Yeh et al., 2001. Prevalence of Campylobacter spp., Escherichia coli and Salmonella serovars in retail chicken, Turkey, pork and beef from the Greater Washington D.C., Area. Applied Environ. Microbiol., 67: 5431-5436.
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