In vitro Effects of Calcium, Magnesium, Mercury and Copper Ions on Neuraminidase of a Nigerian Newcastle Disease Virus Strains


Authors

  • Sunday Blessing Oladele Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
  • King Akpofure Nelson Esievo Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
  • Paul Abdu Department of Surgery and Medicine, Ahmadu Bello University, Zaria, Nigeria
  • Andrew Jonathan Nok Department of Biochemistry,Faculty of Science, Ahmadu Bello University, Zaria, Nigeria

DOI:

https://doi.org/10.3923/ijps.2008.565.569

Keywords:

Cations, effect, neuraminidase and new castle disease virus

Abstract

This study was carried out to determine the effects of cations on neuraminidase during NDV infections in poultry. A total of two hundred samples from dialyzed neuraminidase of NDV Kudu 113 strain were used for the experiment. Fifty samples each were used on four divalent cations, namely calcium (Ca2+), magnesium (Mg2+), mercury (Hg2+) and copper (Cu2+) ions. Neuraminidase activity of NDV Kudu 113 strain was determined in vitro in the presence of these cations, tested as their chloride form by periodate thiobarbituric acid assay method. Neuraminidase activity was also determined in the control samples that were without cations. Out of the four divalent cations examined, Hg2+ induced the highest neuraminidase activity of 75% at the concentration of 0.24M. Magnesium and Ca2+ ions also increased the activity of neuraminidase of NDV Kudu 113 strain in vitro. On the other hand, Cu2+ significantly inhibited the activity of this enzyme by 69.23% at the concentration of 0.47M. It was concluded that Ca2+, Mg2+ and Hg2+ induced increase in the activity of neuraminidase of NDV Kudu 113 strain, while Cu2+ inhibited its activity in vitro. Further studies should be carried out on the roles of these cations in Newcastle disease (ND) and the possible use of Cu2+ in the future as inhibitor of the activity of neuraminidase of NDV in vivo, so as to reduce the menance of ND in poultry.

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Published

2008-05-15

Issue

Vol. 7 No. 6 (2008)

Section

Research Article

License

Copyright (c) 2008 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Oladele, S. B., Esievo, K. A. N., Abdu, P., & Nok, A. J. (2008). In vitro Effects of Calcium, Magnesium, Mercury and Copper Ions on Neuraminidase of a Nigerian Newcastle Disease Virus Strains. International Journal of Poultry Science, 7(6), 565–569. https://doi.org/10.3923/ijps.2008.565.569