Survey for Serum Neuraminidase, Free and Erythrocyte Surface Sialic Acid from Chickens Vaccinated Against Newcastle Disease and the Unvaccinated Chickens Reared under Semi-Intensive Management System


Authors

  • Sunday Blessing Oladele Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
  • Paul Abdu Department of Surgery and Medicine, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
  • Andrew Jonathan Nok Department of Biochemistry, Faculty of Science, Ahmadu Bello University, Zaria, Nigeria
  • King Akpofure Nelson Esievo Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria

DOI:

https://doi.org/10.3923/ijps.2007.933.936

Keywords:

Neuraminidase, newcastle disease virus, sialic acid

Abstract

The study was carried out to determine the levels of neuraminidase, erythrocyte surface and free serum sialic acid concentrations of chickens that were vaccinated against Newcastle Disease (ND) and unvaccinated chickens reared under semi-intensive management system in Zaria metropolis. A total of one hundred chickens, fifty each from vaccinated and unvaccinated flocks, randomly selected from ten backyard poultry farms were used for the study. Neuraminidase activity and concentrations of erythrocyte surface and free serum sialic acids were determined for each chicken, using thiobarbituric acid assay method. The values of Packed Cell Volume (PCV) and antibody titres to Newcastle Disease Virus (NDV) were also determined for each chicken by microhaematocrit and Haemagglutination Inhibition (HI) methods, respectively. The mean neuraminidase activity (4.514±1.70 μmol/min) and free serum sialic acid concentration (0.075±0.03 mg/ml) obtained from the vaccinated chickens were significantly higher (p<0.001) than the corresponding values of 1.870±0.16 µmol/min and 0.031±0.02 mg/ml recorded for the unvaccinated chickens. Similarly, the mean HI antibody titres of log2 5.66±3.56 obtained from the vaccinated chickens was significantly higher (p<0.001) than the values of log2 0.980±0.42 recorded for the unvaccinated chickens. On the other hand, there was no significant difference (p>0.05) between the mean erythrocyte surface sialic acid concentration of 0.045±0.01 mg/ml and 0.040±0.02 mg/ml obtained from vaccinated and unvaccinated chickens, respectively. It was concluded that chickens that were vaccinated against ND had significantly higher levels of neuraminidase activity and free serum sialic acid concentrations than the unvaccinated chickens. Further studies are required to isolate and characterize neuraminidase of field strains of NDV present in the area of study. This could possibly give further insight into the role (s) of this enzyme during vaccination of poultry with NDV vaccines.

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Published

2007-11-15

Issue

Vol. 6 No. 12 (2007)

Section

Research Article

License

Copyright (c) 2007 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Oladele, S. B., Abdu, P., Nok, A. J., & Esievo, K. A. N. (2007). Survey for Serum Neuraminidase, Free and Erythrocyte Surface Sialic Acid from Chickens Vaccinated Against Newcastle Disease and the Unvaccinated Chickens Reared under Semi-Intensive Management System. International Journal of Poultry Science, 6(12), 933–936. https://doi.org/10.3923/ijps.2007.933.936