A Simple Protocol for Amplification of Genes from Inactivated Oil Adjuvant Fowl Cholera Vaccine


Authors

  • P.X. Antony Rajiv Gandhi College of Veterinary and Animal Sciences, Pondicherry, India
  • G.K. Nair College of Veterinary and Animal Sciences, Mannuthy, Kerala, India
  • V. Jayaprakasan College of Veterinary and Animal Sciences, Mannuthy, Kerala, India
  • M. Mini College of Veterinary and Animal Sciences, Mannuthy, Kerala, India
  • S. George College of Veterinary and Animal Sciences, Mannuthy, Kerala, India

DOI:

https://doi.org/10.3923/ijps.2006.623.626

Keywords:

OMPH gene, Pasteurella multocida, restriction enzyme analysis, vaccine strain

Abstract

Pasteurellosis is one of the most contagious disease of avians. Control of pasteurellosis is at present carried out by vaccination with whole-cell bacterins, which confer serotype specific protection. Outermembrane proteins (OMP) of Gram negative bacteria are considered the most important immunogens and have been proved to confer immunity. A simple protocol for amplification of outermembrane protein genes from inactivated bacterial vaccines has been standardized. An attempt has been made to compare the OMP genes of field isolates and vaccine strain.

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Published

2006-06-15

Issue

Vol. 5 No. 7 (2006)

Section

Research Article

License

Copyright (c) 2006 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Antony, P., Nair, G., Jayaprakasan, V., Mini, M., & George, S. (2006). A Simple Protocol for Amplification of Genes from Inactivated Oil Adjuvant Fowl Cholera Vaccine. International Journal of Poultry Science, 5(7), 623–626. https://doi.org/10.3923/ijps.2006.623.626