Genetic Grouping of Nephropathogenic Avian Infectious Bronchitis Virus Isolated in Morocco


Authors

  • M.El Bouqdaoui Virology Laboratory UFR Health and Environment, Faculty of Sciences and Techniques Mohammedia, University Hassan Il, BP 146, Mohammedia (20 650), Morocco
  • R.A. Mhand Virology Laboratory UFR Health and Environment, Faculty of Sciences and Techniques Mohammedia, University Hassan Il, BP 146, Mohammedia (20 650), Morocco
  • H. Bouayoune Virology Department, Laboratory Regional of Analysis and Veterinary Research, Agricultural Ministry, 45 Rue Nichakra Rahal, Casablanca 21 000, Morocco
  • M.M. Ennaji Virology Laboratory UFR Health and Environment, Faculty of Sciences and Techniques Mohammedia, University Hassan Il, BP 146, Mohammedia (20 650), Morocco

DOI:

https://doi.org/10.3923/ijps.2005.721.727

Keywords:

Massachusetts vaccine, nephropathogenic infectious bronchitis virus, RFLP, RT-PCR

Abstract

Thirty Moroccan isolates of infectious bronchitis virus (IBV) recovered from unvaccinated broiler chickens flocks originated from different regions of Morocco between 1997 and 2002, and two references strains Massachusetts and 4/91 (vaccine strains) were classified by reverse transcriptase-polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP) analysis using two restriction enzymes Hae III and Alu1. RFLP patterns of the amplified S1 gene of IBV (1720 bp) digested by two restriction enzymes HaeIII and Alu1 showed that Moroccan isolates of IBV were classified into five genotypes with Three genotypes different from vaccine strains, vaccine protection test showed that Massachusetts type vaccine could not protect chickens against challenge with this three new genotypes.

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Published

2005-08-15

Issue

Vol. 4 No. 9 (2005)

Section

Research Article

License

Copyright (c) 2005 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Bouqdaoui, M., Mhand, R., Bouayoune, H., & Ennaji, M. (2005). Genetic Grouping of Nephropathogenic Avian Infectious Bronchitis Virus Isolated in Morocco. International Journal of Poultry Science, 4(9), 721–727. https://doi.org/10.3923/ijps.2005.721.727