Effects of Diluents, Cryoprotectants, Equilibration Time and Thawing Temperature on Cryopreservation of Duck Semen

Abstract

A series of sequential experiments were carried out to determine optimum diluents, cryoprotectants, equilibration time, and thawing temperature for frozen duck semen in order to set up the commercial semen cryopreservating techniques which could be applied to the conservation of genetic resources, breeding, and commercial production in domestic ducks. In experiment 1, the seven semen extenders were studied to determine efficacy of the diluent on cryopreservation of duck Semen. The result showed that the diluent which contains 0.14% potassium citrate, 1.40% sodium glutamate, 0.98% disodium hydrogen phosphate, 0.21% sodium dihydrogen phosphate, 0.7% glucose, and 0.7% inositol was better than other six semen diluents. In experiment 2, the effects of various concentrations of cryoprotectants including glycerol, dimethyl sulphoxide (DMSO), dimethyl acetamide (DMA), and dimethyl formamide (DMF) on cryopreservation of bird semen were evaluated. The results showed that the cryoprotectant containing 10% DMSO was better than others. The experiment 3 was conducted to determine the effect of equilibration time and thawing temperature on cryopreservation of bird semen. The optimum equilibration time was 15 min and the optimum thawing temperature was 40°C