Probiotic Potential of Lactic Acid Bacteria Isolated from Broiler Chickens in Côte d’Ivoire
DOI:
https://doi.org/10.3923/ijps.2022.119.128Keywords:
Lactic acid bacteria, antibiotics, broiler, gastrointestinal tract, probioticAbstract
Objective: The objective of this study was to characterize and assess lactic acid bacteria isolated from the gastrointestinal tract of broilers for their use in poultry farming as potential probiotic in Côte d'Ivoire. Materials and Methods: For this purpose, 90 colonies of lactic acid bacteria isolated from the crop and cecum of broilers were subjected to several probiotic tests: Thermoresistance, tolerance to acid pH and bile salts, self-aggregation and co-aggregation, hydrophobicity, antibacterial activity and sensitivity to antibiotics. Results: The results of this study showed that out of the 90 isolates 44 were resistant to pH 3 and 0.3% bile salt. Of the 44 isolates, 15 showed high probiotic potential. These isolates belong to the species Pediococcus acidilactici (4), Lactobacillus pentosus (4), Weissella confusa (2) Enterococcus faecium (2), Pediococcus pentosaceus (2) and Enterococcus faecalis (1). The heat map analysis also showed that the species with the best probiotic potential were, Lactobacillus pentosus JK (51 and 55) and Enterococcus faecium JK 96. Conclusion: The species Lactobacillus pentosus JK (51 and 55) and Enterococcus faecium JK 96 could be used for the production of probiotic feed for poultry farming in Côte d'Ivoire thus reducing the use of antibiotics.
References
Jannah, S.N., T.R. Saraswati, D. Handayani and S. Pujiyanto, 2018. Antibacterial activity of lactic acid bacteria isolated from gastrointestinal tract of “Ayam Kampung” chicken against food pathogens. J. Phys.: Conf. Ser., Vol. 1025.
Vondruskova, H., R. Slamova, M. Trckova, Z. Zraly and I. Pavlik, 2010. Alternatives to antibiotic growth promoters in prevention of diarrhoea in weaned piglets: A review. Vet. Med., 55: 199-224.
Kazemi, S.A., H. Ahmadi and M.A.K. Torshizi, 2019. Evaluating two multistrain probiotics on growth performance, intestinal morphology, lipid oxidation and ileal microflora in chickens. J. Anim. Physiol. Anim. Nutr., 103: 1399-1407.
FAO/WHO, 2002. Guidelines for evaluation of probiotics in food. Drafting Guidelines for the Evaluation of Probiotics in Food London, Ontario, Canada,
Dicks, L. and M. Botes, 2010. Probiotic lactic acid bacteria in the gastro-intestinal tract: Health benefits, safety and mode of action. Beneficial Microbes, 1: 11-29.
Park, J.H. and I.H. Kim, 2015. The effects of the supplementation of Bacillus subtilis RX7 and B2A strains on the performance, blood profiles, intestinal Salmonella concentration, noxious gas emission, organ weight and breast meat quality of broiler challenged with Salmonella typhimurium. J. Anim. Physiol. Anim. Nutr., 99: 326-334.
Abdelbasset, A., 2016. Development of a Prebiotic Matrix for the Encapsulation of Bacteriocinogenic Probiotics, Intended for Animal Feed: From Physicochemistry to Biopharmacy. PhD Theses, Laval University, Canada.
Badis, A., N. Laouabdia-Sellami, D. Guetarni, M. Kihal and R. Ouzrout, 2005. Phenotypic characterization of lactic bacteria isolated from raw goat milk from two local goat populations "Arabia and Kabyl. Sci. Technol. C, Biotechnol., 23: 30-37.
Ramos, C.L., L. Thorsen, R.F. Schwan and L. Jespersen, 2013. Strain-specific probiotics properties of Lactobacillus fermentum, Lactobacillus plantarum and Lactobacillus brevis isolates from Brazilian food products. Food Microbiol., 36: 22-29.
Handa, S. and N. Sharma, 2016. In vitro study of probiotic properties of Lactobacillus plantarum F22 isolated from chhang-A traditional fermented beverage of Himachal Pradesh, India. J. Genet. Eng. Biotechnol., 14: 91-97.
Taheri, H.R., H. Moravej, F. Tabandeh, M. Zaghari and M. Shivazad, 2009. Screening of lactic acid bacteria toward their selection as a source of chicken probiotic. Poult. Sci., 88: 1586-1593.
Kos, B., J. Suskovic, S. Vukovic, M. Simpraga, J. Frece and S.J. Matosic, 2003. Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. J. Appl. Microbiol., 94: 981-987.
Arici, M., B. Bilgin, O. Sagdic and C. Ozdemir, 2004. Some characteristics of Lactobacillus isolates from infant faeces. Food Microbiol., 21: 19-24.
Bauer, A.W., W.M.M. Kirby, J.C. Sherris and M. Turck, 1966. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol., 45: 493-496.
CASFM, 2018. Comité de l’antibiogramme de la Société Française de Microbiologie. https://www.sfm-microbiologie.org/wp-content/uploads/2018/12/CASFMV2_SEPTEMBRE2018.pdf
Reuben, R.C., P.C. Roy, S.L. Sarkar, R.U. Alam and I.K. Jahid, 2019. Isolation, characterization, and assessment of lactic acid bacteria toward their selection as poultry probiotics. BMC Microbiol., Vol. 19.
Konings, W.N., J.S. Lolkema, H. Bolhuis, H.W. van Veen, B. Poolman and A.J.M. Driessen, 1997. The role of transport processes in survival of lactic acid bacteria, Energy transduction and multidrug resistance. Antonie van Leeuwenhoek, 71: 117-128.
Lin, J., O. Sahin, L.O. Michel and Q. Zhang, 2003. Critical role of multidrug efflux pump cmeabc in bile resistance and In vivo colonization of Campylobacter jejuni. Infec. Immun., 71: 4250-4259.
García-Hernández, Y., T. Pérez-Sánchez, R. Boucourt, J.L. Balcázar and J.R. Nicoli et al., 2016. Isolation, characterization and evaluation of probiotic lactic acid bacteria for potential use in animal production. Res. Vet. Sci., 108: 125-132.
Shokryazdan, P., R. Kalavathy, C.C. Sieo, N.B. Alitheen, J.B. Liang and M.F. Jahromi, 2014. Isolation and characterization of Lactobacillus strains as potential probiotics for chickens. Pertanika J. Trop. Agric. Sci., 37: 141-157.
Onifade, A.A., 1997. Growth performance, carcass characteristics, organs measurement and haematology of broiler chickens fed a high fibre diet supplemented with antibiotics or dried yeast. Nahrung, 41: 370-374.
Ehrmann, M.A., P. Kurzak, J. Bauer and R.F. Vogel, 2002. Characterization of lactobacilli towards their use as probiotic adjuncts in poultry. J. Applied Microbiol., 92: 966-975.
Jannah, S.N., A. Dinoto, K.G. Wiryawan and I. Rusmana, 2014. Characteristics of lactic acid bacteria isolated from gastrointestinal tract of cemani chicken and their potential use as probiotics. Media Peternakan, 37: 182-189.
Gonzalez, L., H. Sandoval, N. Sacristan, J.M. Castro, J.M. Fresno and M.E. Tornadijo, 2007. Identification of lactic acid bacteria isolated from Genestoso cheese throughout ripening and study of their antimicrobial activity. Food Control, 18: 716-722.
Nallala, V., V. Sadishkumar and K. Jeevaratnam, 2017. Molecular characterization of antimicrobial Lactobacillus isolates and evaluation of their probiotic characteristics in vitro for use in poultry. Food Biotechnol., 31: 20-41.
Kizerwetter-Swida, M. and M. Binek, 2005. Selection of potentially probiotic Lactobacillus strains towards their inhibitory activity against poultry enteropathogenic bacteria. Polish J. Microbiol., 54: 287-294.
Oyewole, O.F., C.O. Maria, P.S. Tope and O.O. Funmi, 2018. In vitro study of potential probiotic lactic acid Bacteria isolated from the gut of chickens in Abeokuta, Nigeria. Alexandria J. Vet. Sci., 58: 73-84.
Shamsudin, W.N.F., S.S. Loo, H.W. Ho, N. Abdullah, W.Z. Saad and K.L. Wan, 2019. Probiotic properties of Lactobacillus isolates from chicken intestines. J. Bio. Microbiol. Biotech., 7: 8-13.
Denev, S., E. Chevaux and V. Demey, 2013. Efficacy of the Probiotic Pediococcus acidilactici on Laying Hens' Performances. Actes des 10èmes Journées de la Recherche Avicole et Palmipèdes à Foie Gras du 26 au 28 mars, 2013, La Rochelle, France. March 26-28 Institut Technique de l'Aviculture, 943-946.
Olajugbagbe, T.E., O.E. Elugbadebo and B.O. Omafuvbe, 2020. Probiotic potentials of Pediococuss acidilactici isolated from wara; a Nigerian unripened soft cheese. Heliyon, Vol. 6.
Ayyash, M., B. Abu-Jdayil, A. Olaimat, G. Esposito and P. Itsaranuwat et al., 2020. Physicochemical, bioactive and rheological properties of an exopolysaccharide produced by a probiotic Pediococcus pentosaceus M41. Carbohydr. Polym., Vol. 229.
Jiang, S., L. Cai, L. Lv and L. Li, 2021. Pediococcus pentosaceus, a future additive or probiotic candidate. Microb. Cell Fact., Vol. 20.
Franz, C.M.A.P., M. Huch, H. Abriouel, W. Holzapfel and A. Galvez, 2011. Enterococci as probiotics and their implications in food safety. Int. J. Food Microbiol., 151: 125-140.
Zommiti, M., M. Cambronel, O. Maillot, M. Barreau and K. Sebei et al., 2018. Evaluation of probiotic properties and safety of Enterococcus faecium isolated from artisanal Tunisian meat “Dried Ossban”. Front. Microbiol., Vol. 9.
Hoque, M.Z., F. Akter, K.M. Hossain, M.S.M. Rahman, M.M. Billah and K.M.D. Islam, 2010. Isolation, identification and analysis of probiotic properties of Lactobacillus spp. from selective regional yoghurts. World J. Dairy Food Sci., 5: 39-46.
Jose, N.M., C.R. Bunt and M.A. Hussain, 2015. Comparison of microbiological and probiotic characteristics of lactobacilli isolates from dairy food products and animal Rumen contents. Microorganisms, 3: 198-212.
Kim, D.-H and B. Austin, 2008. Characterization of probiotic Carnobacteria isolated from rainbow trout (Oncorhynchus mykiss) intestine. Lett. Appl. Microbiol., 47: 141-147.
Downloads
Published
Issue
Section
License
Copyright (c) 2022 The Author(s)
This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
