Effects of Oral Administration of Encapsulated-Leucine on Amino Acid and Plasma Metabolite Profiles in Broiler Chicks During the Starter Phase
DOI:
https://doi.org/10.3923/ijps.2020.252.256Abstract
Background and Objective: An abundant studies show that leucine (Leu) acts as an anabolic agent that stimulates skeletal muscle growth in human as well as in animals. However, the effect of encapsulated leucine (CL) supplementation on growth performance of broiler chickens has not been evaluated. Therefore, the aim of this study was to determine initial metabolic responses to CL supplementation on food intake, plasma metabolites and branched chain amino acid concentration in 7-day-old broiler chicks. Materials and Methods: A total of 24 chicks were randomly assigned to the following treatments: (1) Control 4 h (C), (2) Free leucine 4h (L) and (3) Encapsulated-leucine 4h (CL). After 6 h of fasting, chicks were given a bolus of oral injection of distilled water, free leucine (6 mmol/10 mL kg–1 BW) or encapsulated-leucine (6 mmol/10 mL kg–1 BW). Immediately after the injection, chicks were allowed free access to a commercial starter diet for 1 h. Blood collections were obtained 4 h after the oral injection. Food intake, total glucose, total cholesterol, triacylglycerol, plasma leucine levels and the activity of glutamic oxaloacetic transaminase (GOT) were measured. Results: Food intake, glucose, total cholesterol and triacylglycerol levels were not affected by Leu supplementation. At 4 h, GOT levels were greater (p<0.05) in the CL group than that of the Leu groups. At 4 h, although plasma Leu levels were similar in all groups, Valine levels were lower (p<0.005) in the C and CL groups than that of the Leu group and isoleucine levels were lower (p<0.001) in the CL group than that of the C and Leu groups. Conclusion: The results of this study suggest that an oral administration of CL caused prolonged leucine-induced anabolic effects that may be beneficial for growth. Our observations also pave the way for studying the long-term effects of CL supplementation on performance of broiler chicks.
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