Dosage Effects of Lactococcus lactis ssp. lactis 2 as a Probiotic on the Percentage of Carcass, Abdominal Fat Content and Cholesterol Level in Broilers
DOI:
https://doi.org/10.3923/ijps.2018.100.105Keywords:
Abdominal fat, broiler, carcass, cholesterol, Lactococcus lactis ssp. lactis 2Abstract
Objective: This study was conducted to determine the dosage effects of the lactic acid bacteria (LAB) Lactococcus lactis ssp. lactis 2 as a probiotic on the percentage of carcass, abdominal fat content and cholesterol level in broilers. Methodology: Lactococcus lactis ssp. lactis 2 was isolated from broiler faeces. The microbe count was 1.3×108 CFU g–1. The treatments in this study consisted of the following groups, P0: Without administration of the probiotic, P1: With the administration of 0.01% zinc bacitracin antibiotic, P2: With the administration of Lactococcus lactis ssp. lactis 2 probiotic at a dose of 0.5% (6.5×108 CFU kg–1), P3: With the administration of Lactococcus lactis ssp. lactis 2 probiotic at a dose of 1% (1.3×109 CFU kg–1) and P4: With the administration of Lactococcus lactis ssp. lactis 2 probiotic at a dose of 1.5% (1.95×109 CFU kg–1). Results: This study showed that administration of the LAB Lactococcus lactis ssp. lactis 2 with a 0.5% (6.5×108 CFU kg–1), 1% (1.3×109 CFU kg–1) and 1.5% (1.95×109 CFU kg–1) dose did not influence abdominal fat content, however, probiotic administration reduced cholesterol in breast meat and thigh meat and reduced fat in breast meat compared to the antibiotic and control treatments. Administration of Lactococcus lactis ssp. lactis 2 probiotic at a 1.5% dose (1.95×109 CFU kg–1) resulted in a higher percentage of carcass and lower percentage of thigh meat cholesterol compared to the 0.5% (6.5×108 CFU kg–1) and 1% (1.3×109 CFU kg–1) doses. Conclusion: The administration of Lactococcus lactis ssp. lactis 2 did not affect abdominal fat content but reduced breast meat cholesterol and thigh meat cholesterol. The 1.5% dose (1.95×109 CFU kg–1) produced a higher percentage of carcass and lower thigh meat cholesterol than did the other doses.
References
Purwadaria, T., I.P. Kompiang, J. Darma and Supriyati, 2003. Screening of bacteria resistant to antibiotics for use as poultry probiotics. JITV., 8: 151-156.
Wahyudi, A. and L. Hendraningsih, 2007. Probiotics, Concepts and Application of Ruminants. UMM Press, Malang.
Daud, M., W.G. Piliang and I.P. Kompiang, 2007. Percentage and quality of broiler chicken which given probiotic and prebiotic in Ransum. JITV., 12: 167-174.
Afriani, H., 2002. Effect of culture dosage of Bacillus spp. and Saccharomyces cerevisiae as a probiotic to the performance, fat content and cholesterol carcass of broiler chickens. Master's Thesis, University of Pajajaran, Bandung.
Mujnisa, A., 2012. Potential lactic acid bacteria isolation result from broiler feces as feed additive to broiler productivity. Master's Thesis, Faculty of Animal Science, University of Hasanuddin, Makassar, Indonesia.
AOAC, 1995. Association of Official Agricultural Chemists. 16th Edn., Association of Official Analytical Chemists, Washington DC, USA, ISBN: 9780935584547.
Bell, D.D. and W.J. Weaver, 2002. Commercial Chicken Meat and Egg Production. 5th Edn., Kluwer Academic Publishers, USA.
Adriani, L., T. Widjastuti and R. Dermawan, 2010. The effect of probiotic supplemented ration on broiler abdominal fatty content and final weight. Lucrari Stiinfifice-Universitatea Stiinte Agricole Medicina Veterinara Seria Zootehnie, 53: 94-96.
Walker, D.K. and S.E. Gilliland, 1993. Relationship among bile tolerance, bile salt deconjugation and assimilation of cholesterol by Lactobacillus acidophilus. J. Dairy Sci., 76: 956-961.
Gilliland, S.E., 1990. Bacterial Starter Culture for Food. 5th Edn., CRC Press Inc., Florida, USA.
Harmayani, E., E.S. Rahayu and Y. Marsono, 2003. Influence of Giving of Lactobacillus sp. Dad13 on Sprague dawley Lipid Profile Who were Given a Hypercholesterol Diet. PATPI., Yogyakarta, Indonesia.
Liong, M.T. and N.P. Shah, 2005. Bile salt deconjugation ability, bile salt hydrolase activity and cholesterol co-precipitation ability of lactobacilli strains. Int. Dairy J., 15: 391-398.
Sanders, M.E., 2000. Considerations for use of probiotic bacteria to modulate human health. J. Nutr., 130: 384S-390S.
Basmacioglu, H. and M. Ergul, 2005. Research on the factors affecting cholesterol content and some other characteristics of eggs in laying hens: The effects of genotype and rearing system. Turk. J. Vet. Anim. Sci., 29: 157-164.
Khaksefidi, A. and S. Rahimi, 2005. Effect of probiotic inclusion in the diet of broiler chickens on performance, feed efficiency and carcass quality. Asian Aust. J. Anim. Sci., 18: 1153-1156.
Endo, T. and M. Nakano, 1999. Influence of a probiotic on productivity, meat components, lipid metabolism, caecal flora and metabolites and raising environment in broiler production. Anim. Sci. J., 70: 207-218.
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