Genetic Differentiation in the Oh-Shamo (Japanese Large Game) Breed of Chickens Assessed by Microsatellite DNA Polymorphisms
DOI:
https://doi.org/10.3923/ijps.2014.319.322Keywords:
Genetic differentiation, genetic diversity, Japanese large game, native Japanese chickens, Oh-Shamo, microsatelliteAbstract
The Oh-Shamo is a native Japanese chicken breed that has been improved for cock fighting purpose for approximately 400 years. Two types of Oh-Shamo have been created: one type concentrates on offence and the other type is specialized for defense. In addition to these Oh-Shamo types for cock fighting, commercial stocks have been established to produce brand meat in Japan. In this article, we revealed the genetic differentiation among Oh-Shamo populations based on the difference in the use and the manner of fighting, as well as genetic diversity in these populations. MNA, AR, HO and HE were 2.55-4.03, 2.52-3.75, 0.420-0.577 and 0.422-0.559, respectively, throughout both fighting- and meat-purpose populations. There was a tendency for fighting-type Oh-Shamo populations to show higher genetic diversity. Inbreeding was not observed in both fighting-purpose and meat-purpose Oh-Shamo populations. Neighbor-joining tree topology based on DPS genetic distance clearly separated fighting-purpose Oh-Shamo from meat-purpose Oh-Shamo. Thus, commercial Oh-Shamo for meat production is genetically distinct from the original Oh-Shamo used for cock fighting. On the other hand, no conspicuous genetic differentiation was observed between offence- and defense-types of fighting-purpose Oh-Shamo.
References
Bowcock, A.M., A. Ruiz-Linares, J. Tomfohrde, E. Minch, J.R. Kidd and L.L. Cavalli-Sforza, 1994. High resolution of human evolutionary trees with polymorphic microsatellites. Nature, 368: 455-457.
FAO., 2004. Secondary guidelines for development of national farm animal genetic resources management plans. Measurement of Domestic Animal Diversity (Mo-DAD): Recommended microsatellite markers. FAO, Rome, Italy.
Goudet, J., 1995. FSTAT (version 1.2): A computer program to calculate F-statistics. J. Heredity, 86: 485-486.
Oka, T., Y. Takahashi, K. Nomura, H. Hanada, T. Amano and F. Akishinonomiya, 2010. Genetic diversity and population structure of Shamo and its related breeds of Japanese native chicken based on microsatellite DNA polymorphisms. J. Agric. Sci., Tokyo Univ. Agric., 55: 211-218.
Osman, S.A.M., M. Sekino, A. Nishihata, Y. Kobayashi and W. Takenaka et al., 2006. The genetic variability and relationships of Japanese and foreign chickens assessed by microsatellite DNA profiling. Asian-Aust. J. Anim. Sci., 19: 1369-1378.
Saitou, N. and M. Nei, 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol., 4: 406-425.
Sambrook, J. and D.W. Russell, 2001. Molecular Cloning: A Laboratory Manual. 3rd Edn., Cold Spring Harbor Laboratory Press, New York, USA., ISBN-13: 9780879695774, Pages: 2344.
Tsudzuki, M., 2003. Japanese Native Chickens. In: The Relationship between Indigenous Animals and Humans in APEC Region, Chang, H.L. and Y.C. Huang (Eds.). The Chinese Society of Animal Science, Taiwan, Tainan, pp: 91-116.
Weir, B.S. and C.C. Cockerham, 1984. Estimating F-statistics for the analysis of population structure. Evolution, 38: 1358-1370.
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