Association Between Candidate Genes of Innate Immunity, Gallinacin Genes and Resistance to Marek’s Disease in Chicken


Authors

  • H.A. Yacoub Department of Cell Biology, National Research Center, Dokki, Giza, Egypt
  • A. Galal Department of Poultry Production, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
  • M.M. Fathi Department of Poultry Production, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
  • S.A. El Fiky Department of Cell Biology, National Research Center, Dokki, Giza, Egypt
  • H.A.I. Ramadan Department of Cell Biology, National Research Center, Dokki, Giza, Egypt

DOI:

https://doi.org/10.3923/ijps.2011.656.661

Keywords:

Gallinacin, genes, innate immunity, Marek`s disease, resistance

Abstract

Gallinacins are antimicrobial peptides that play a significant role in innate immunity in chicken. The aim of this study was to determine the relationship between gallinacin genes and resistance to Marek's disease and to predict whether the amino acids substitutions lead to produce new phenotypes. We used in current study two inbred White Leghorn Lines 6 and 7. We examined gallinacins genes (3-5 and 10) by sequenced a 2.29 kb in two directions from two inbred lines (6 and 7). A total of 10 SNPs were identified within the sequenced regions. This equates to an SNP rate of 4.36 SNPs/kb, nearly to the previously reported 5 SNPs/kb across the entire chicken genome. The current study showed that the gallinacin genes are polymorphic because there are many (SNPs) in both inbred lines of White Leghorn chickens and some of these SNPs are nonsynonymous and others are synonymous. We are concluded that a new chromosomal region with effects on the response to Marek's disease in chickens was characterized in this study. Within this region, the SNPs in the gallinacin candidate genes could potentially be used in a marker assisted selection program to enhance the response to Marek's disease. Analysis of the gallinacin genes in the protective pathways of disease resistance has also opened the possibilities for therapeutic strategies using endogenous antimicrobial peptides.

References

Bar-Shira, E. and A. Friedman, 2006. Development and adaptations of innate immunity in the gastrointestinal tract of the newly hatched chick. Dev. Comp. Immunol., 30: 930-941.

Calnek, B.W. and R.L. Witter, 1997. Neoplastic Diseases/Marek's Disease. In: Diseases of Poultry, Calnek, F.W., H.J. Barnes, C.W. Beard, L.R. McDougald and Y.M. Saif (Eds.). 10th Edn., Iowa State University Press, Ames, IA., USA., pp: 369-413.

Emara, M.G. and H. Kim, 2003. Genetic markers and their application in poultry breeding. Poult. Sci., 82: 952-957.

Hasenstien, J.R., G. Zhang and S.J. Lamont, 2006. Analysis of five gallinacin genes and the Salmonella entrica serovar enteritidis response in poultry. Infect. Immunity, 74: 3375-3380.

Ma, D.Y., S.W. Liu, Z.X. Han, Y.J. Li and A.S. Shan, 2008. Expression and characterization of recombination gallinacin-9 and gallinacin-8 in Escherichia coli. Protein Exp. Purif., 58: 284-291.

Cheng, H., M. Niikura, T. Kim, W. Mao and K.S. MacLea et al., 2008. Using integrative genomics to elucidate genetic resistance to Marek's disease in chickens. Dev. Biol., 132: 365-372.

Pazderka, J., B.M. Longgeneker, G.R.J. Law, H.A. Stone and R.F. Ruth, 1975. Histocompatibility of chicken populations selected for resistance to Marek's disease. Immunogenetics, 2: 93-100.

Purchase, H.G., 1985. Clinic Disease and its Economic Impact. In: Marek's Disease: Scientific Basis and Methods of Control, Payne, L.N. (Ed.). Martinus NKjhoff Publishing, Boston, USA., ISBN-13: 9780898387308, pp: 174.

Risch, N. and K. Merikangas, 1996. The future of genetic studies of complex human diseases. Science, 273: 1516-1517.

Satchell, D.P., T. Sheynis, Y. Shirafuji, S. Kolusheva, A.J. Quellette and R. Jelinek, 2003. Interaction of mouse Paneth cell α-defensins and α-defensin precursors with membranes. Prosegment inhibition of peptide association with biomimetic membranes. J. Biol. Chem., 278: 13838-13846.

Sunyaev, S., V. Ramensky and P. Brok, 2000. Towards a structure basis of human nonsynonymous single nucleotide polymorphisms. Trends Genet., 16: 198-200.

Vallejo, R.L., L.D. Bacon, H.C. Liu, R.L. Witter, M.A. Gronenen, J. Hille and H.H. Cheng, 1997. Genetic mapping of quantitative trait loci affecting susceptibility to Marek's disease virus induced tumors in F2 intercross chickens. Genetics, 148: 349-360.

Wang, Z. and J. Moult, 2001. SNPs, protein structure and disease. Hum. Mutat., 17: 263-270.

Witter, R.L. and H.D. Hunt, 1994. Poultry vaccines of the future. Poult. Sci., 73: 1087-1093.

Wong, G., B. Liu, J. Wang, Y. Zhang and X. Yang et al., 2004. A genetic variation map for chicken with 2.8 million single-nucleotide polymorphisms. Nature, 432: 717-722.

Xiao, Y., A.L. Hughes, J. Ando, Y. Matsuda, J.F. Cheng, D. Skinner-Noble and G. Zhang, 2004. A genome-wide screen identifies a single beta defensins gene cluster in the chicken: Implications for the origin and evolution of mammalian defensins. BMC Genomics, Vol. 5.

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Published

2011-07-15

Issue

Vol. 10 No. 8 (2011)

Section

Research Article

License

Copyright (c) 2011 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Yacoub, H., Galal, A., Fathi, M., El Fiky, S., & Ramadan, H. (2011). Association Between Candidate Genes of Innate Immunity, Gallinacin Genes and Resistance to Marek’s Disease in Chicken. International Journal of Poultry Science, 10(8), 656–661. https://doi.org/10.3923/ijps.2011.656.661

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