Antimicrobial Screening of Commercial Eggs and Determination of Tetracycline Residue Using Two Microbiological Methods
DOI:
https://doi.org/10.3923/ijps.2010.959.962Keywords:
Antimicrobials, commercial eggs, residues, tetracyclineAbstract
Thirty commercial layer farms were surveyed to obtain information on drug use and to screen eggs for antimicrobial residues. Tetracycline residue was specifically tested using a commercial test kit. The study indicated that up to a third of farmers (33.3%) were not adhering to the recommendation on drug use and thereby allowing drug residues in eggs. Of the 900 commercial eggs screened, 3.6% tested positive for antimicrobial residues but only 0.1% tested positive for tetracycline residue. The low level of tetracycline residue detected in this study is an indication of the declining use of this antibiotic in the poultry industry perhaps due to the increasing availability of cheaper alternatives. There is a need for strict regulation of veterinary drug in order to guarantee food safety and effective use. To ensure compliance with drug use in Nigeria, routine surveillance must be conducted using simple detection methods.
References
Shitandi, A. and K. Gathoni, 2005. Evaluation of the Bacillus calidolactis plate for post screening assay of β-lactam antimicrobial residues in Kenyan dairies. Food Control, 16: 227-230.
Baker, B. and D. Leyland, 1983. The chemistry of tetracycline antibiotics. J. Chromatogr., 24: 30-35.
Blomquist, L. and A. Hannigren, 1966. Fluorescent technique for distribution studies of tetracyclines. Biochem. Pharm., 188: 215-219.
Droumev, D.R., 1983. Review of antimicrobial growth promoting agents available. Vet. Res. Commun., 7: 85-99.
Johnston, A., 1998. Use of antimicrobial drugs in veterinary practice. Br. Med. J., 317: 665-667.
Kabir, J., J.U. Umoh and V.J. Umoh, 1999. Public health awareness and health concern for veterinary drug residues in meat in Nigeria. Health Hyg., 20: 20-24.
Kabir, J., V.J. Umoh, E. Audu-Okoh, J.U. Umoh and J.K.P. Kwaga, 2004. Veterinary drug used in poultry farms and determination of antimicrobial drug residues in commercial eggs and slaughtered chicken in Kaduna State, Nigeria. Food Control, 15: 99-105.
Kaneene, J.B. and R. Miller, 1997. Problems associated with drug residues in beef from feeds and therapy. Rev. Sci. Tech., 16: 694-708.
Lee, M.H., H.J. Lee and P.D. Ryu, 2001. Public health risks: Chemical and antibiotic residues-review. Asian Australas. J. Anim. Sci., 14: 402-413.
Pavlov, A.I., L. Lashev, I. Vachin and V. Rusev, 2008. Residues of antimicrobial drugs in chicken meat and offals. Trakia J. Sci., 6: 23-25.
Salehzadeh, F., R. Madani, A. Salehzadeh, N. Rokni and F. Golchinefar, 2006. Oxytetracycline residue in chicken tissues from Tehran slaughterhouses in Iran. Pak. J. Nutr., 5: 377-381.
Shahid, M.A., M. Siddique, Sajjad-Ur-Rehman, S. Hameed and A. Hussain, 2007. Evaluation of a microbiological growth inhibition assay as a screening test for the presence of antibiotic residues in poultry meat. Am. J. Food Technol., 2: 457-461.
Stead, S.L., M. Caldow, A. Sharma, H.M. Ashwin, M. Sharman, A. De-Rijk and J. Stark, 2007. New method for the rapid identification of tetracycline residues in foods of animal origin-using the premi-test in combination with a metal ion chelation assay. Food Addit. Contam., 24: 583-589.
Stowe, M., D. Terhune and P. Wilmore, 1980. A survey of use and misuse of tetracyclines. USA. Anim. Health Meet., 81: 331-338.
WHO, 1989. Evaluation of certain veterinary drug residues in food: Thirty fourth Report of the Joint FAO/WHO Expert Committee on Food Additives. WHO Technical Report Series, No. 788, Geneva.
Downloads
Published
Issue
Section
License
Copyright (c) 2010 Asian Network for Scientific Information

This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.