Differential Expression of IL-6 and IGF-II in Guinea Fowl and Chicken
DOI:
https://doi.org/10.3923/ijps.2010.756.760Keywords:
Differential expression, guinea fowl, IGF-II, IL-6Abstract
Differential expression of IL-6 and IGF-II genes were studied in guinea fowl and broiler chicken using semi-quantitative analysis. A 219 bp fragment of IL-6 and 215 bp fragment of IGF-II were amplified in guinea fowl and broiler chicken using chicken specific primers. Semi-quantitative analysis revealed the adjusted Integral Density of 0.853 and 0.051 for IL-6 band in guinea fowl and broiler chicken respectively, revealing 16.62 fold higher IL-6 mRNA expression in LPS induced PBMCs from guinea fowl as compared to that from broiler. However, adjusted Integral Density of IGF-II band was 0.082 and 1.106 for IGF-II band in guinea fowl and broiler chicken respectively, which revealed 13.43 fold increase in IGF-II mRNA expression in LPS induced PBMCs in broiler chicken as compared to that in guinea fowl. Hence, guinea fowl showed higher expression of pro-inflammatory cytokine (IL-6) and lower expression of IGF-II in comparison to broiler chicken. These findings were as per expectation in view of much higher immuno-competence and lower growth rate in guinea fowl in comparison to chicken.
References
Cunningham, C.H., 1971. Virologia Practica. 6th Edn., Acribia, Zaragoza, Pages: 260.
Erickson, G.A., C.J. Mare, G.A. Gustafson, L.D. Miller, S.J. Proctor and E.A. Carbrey, 1977. Interaction between viscerotropic velogenic Newcastle disease virus and pet birds of six species I. Clinical and serological responses and viral excretion. Avian Dis., 21: 642-654.
Hofstad, M.S., 1951. A quantitative study of Newcastle disease virus in tissues of infected chickens. Am. J. Vet. Res., 12: 334-339.
Kaleta, E.F. and C. Baldauf, 1988. Newcastle Disease in Free-Living and Pets Birds. In: Newcastle Disease, Alexander, D.J. (Ed.). Kluwer Academic Publishers, Boston, MA., USA., pp: 197-246.
Lendon, A.H., 1973. Australian Parrots in Field and Aviary. 2nd Edn., Angus and Robertson, Sydney, pp: 302-307.
McFerran, J.B. and R. Nelson, 1971. Some properties of an avirulent Newcastle disease virus. Arch. Virol., 34: 64-74.
Winterfield, R.W., C.L. Goldman and E.H. Seadale, 1957. Newcastle disease immunization studies: Vaccination of chickens with B1, F and LaSota strains of Newcastle disease virus administered through the drinking water. Poult. Sci., 36: 1076-1088.
Aitken, I., W.H. Allan, R.M. Biggs, R.I. Gordon and F.T.W. Jordan, 1977. Newcastle Disease. In: Poultry Diseases, Gordon, R.F. (Ed.). Bailliere Tindall, London, pp: 81-94.
Bayyari, G.R., W.E. Huff, N.C. Rath, J.M. Balog and L.A. Newberry et al., 1997. Effect of the genetic selection of turkeys for increased body weight and egg production on immune and physiological responses. Poult. Sci., 76: 289-296.
Beccavin, C., B. Chevalier, L.A. Cogburn, J. Simon and M.J. Duclos, 2001. Insulin-like growth factors and body growth in chickens divergently selected for high or low growth rate. J. Endocrinol., 168: 297-306.
Burnside, J. and L.A. Cogburn, 1992. Developmental expression of hepatic growth hormone receptor and insulin-like growth factor-I mRNA in the chicken. Mol. Cell. Endocrinol., 89: 91-96.
Cheng, H.H. and S.J. Lamont, 2008. Genetics of Disease Resistance. In: Diseases of Poultry, Saif, Y.M., A. Fadly, J. Glisson, I. McDonald, L. Nolan and D. Swayne (Eds.). 12th Edn. Blackwell Publication, UK., pp: 59-72.
Chiang, H.I., C.L. Swaggerty, M.H. Kogut, S.E. Dowd, X. Li, I.Y. Pevzner and H. Zhou, 2008. Gene expression profiling in chicken heterophils with Salmonella enteritidis stimulation using a chicken 44 K Agilent microarray. BMC Genom., 9: 526-526.
Duclos, M.J., 2005. Insulin-like growth factor-I (IGF-1) mRNA levels and chicken muscle growth. J. Physiol. Pharmacol., 56: 25-35.
Ferro, P.J., C.L. Swaggerty, P. Kaiser, I.Y. Pevzner and M.H. Kogut, 2004. Heterophils isolated from chickens resistant to extra-intestinal Salmonella enteritidis infection express higher levels of pro-inflammatory cytokine mRNA following infection than heterophils from susceptible chickens. Epidemiol. Infect., 132: 1029-1037.
Jones, J.I. and D.R. Clemmons, 1995. Insulin-like growth factors and their binding proteins: Biological actions. Endocrinol. Rev., 16: 3-34.
Kajimoto, Y. and P. Rotwein, 1989. Structure and expression of a chicken insulin-like growth factor I precursor. Mol. Endocrinol., 3: 1907-1913.
Kikuchi, K., F.C. Buonomo, Y. Kajimoto and P. Rotwein, 1991. Expression of insulin-like growth factor-I during chicken development. Endocrinology, 128: 1323-1328.
Kougt, M.H., E.D. Mcgrude, B.M. Hargis, D.E. Corrier and J.P. Deloach, 1994. Characterization of the pattern of inflammatory cell influx in chicks following the intraperitoneal administration of line Salmonella enteritidies-immune lymphokines. Poult. Sci., 74: 8-17.
Lamont, S.J., M.H. Pinard-van der Laan, A. Cahaner, J.J. van der Poel and H.K. Parmentier, 2003. Selection for Disease Resistance: Direct Selection on the Immune Response. In: Poultry Genetics, Breeding and Biotechnology, Muir, W.M. and S.E. Aggrey (Eds.). Chapter 22, CABI Publishing, Oxon, UK., ISBN-13: 9780851998459, pp: 399-418.
Muir, W.M., G.K. Wong, Y. Zhang, J. Wang and M.A. Groenen et al., 2008. Genome-wide assessment of worldwide chicken SNP genetic diversity indicates significant absence of rare alleles in commercial breeds. Proc. Natl. Acad. Sci. USA., 105: 17312-17317.
Nestor, K.E., Y.M. Saif, J. Zhu and D.O. Noble, 1996. Variation in resistance to Pasteurella multocida among Turkey lines. Poult. Sci., 75: 1161-1163.
Redmond, S.B., P. Chuammitri, B.C. Andreasen, D. Palic and J.S. Lamont, 2009. Chicken heterophils from commercially selected and non-selected genetic lines express cytokines differently after in vitro exposure to Salmonella enteritidis. Vet. Immunol. Immunopathol., 132: 129-134.
Scanes, C.G., E.A. Dunnington, F.C. Buonomo, D.J. Donoghue and P.B. Siegel, 1989. Plasma concentrations of insulin like growth factors (IGF-I and IGF-II) in dwarf and normal chickens of high and low weight selected lines. Growth Dev. Aging, 53: 151-157.
Schneider, K., R. Klaas, B. Kaspers and P. Staeheli, 2001. Chicken interleukin-6. cDNA structure and biological properties. Eur. J. Biochem., 268: 4200-4206.
Shook, G.E., 1989. Selection for disease resistance. J. Dairy Sci., 72: 1349-1362.
Swaggerty, C.L., M.H. Kogut, P.J. Ferro, L. Rothwell, I.Y. Pevzner and P. Kaiser, 2004. Differential cytokine mRNA expression in heterophils isolated from Salmonella-resistant and-susceptible chickens. Immunology, 113: 139-148.
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