The Influence of Supplemental Multi-enzyme Feed Additive on the Performance, Carcass Characteristics and Meat Quality Traits of Broiler Chickens
DOI:
https://doi.org/10.3923/ijps.2010.126.133Keywords:
Broilers, feed additive, meat quality, multienzyme, performanceAbstract
A study was conducted to investigate the effect of adding a commercial multienzyme feed additive (Tomoko, Biogenkoji Research Institute, Japan) on the performance of broilers. Four isoenergetic and isontirogenous diets consisting of control diet without enzyme (Con) and three test diets supplemented with Tomoko at 250 (T250), 500 (T500) and 750 (T750) g/tonne of feed were used for starter, grower and finisher phases. Each diet was offered to 10 replicates of 40 one-day-old straight-run Lohmann broiler chicks (n = 1600) in a randomized complete block design (10 blocks of 4 diets each). Data was analyzed using mixed procedure of SAS (repeated measures analysis) for a randomized complete block design, with level of significance set at p = 0.05. Enzyme used in the study was authenticated by the supplier to have minimum level of acidic protease (10,000 U/g), alpha-amylase (40 U/g), pectinase (30 U/g), phytase (10 U/g), glucoamylase (5 U/g) and cellulase (4 U/g). Enzyme supplementation had no significant effect on Feed Intake (FI) at 21 d, while at 42 d birds fed T250 and Con diets significantly consumed more feed than T500 and/or T750. No significant differences were observed for Feed Conversion Ratio (FCR). Body Weight (BW) and Body Weight Gain (BWG) were significantly higher (p<0.05) for birds fed Con diet at 42 d. Carcass characteristics showed no significant effects on whole carcass weight and/or dressing percent and weight and percent of breast, thighs and wings. Enzyme supplemented diets significantly (p<0.05) increased liver percent in contrast to Con diet, while no significant differences were reported for heart, gizzard and abdominal fat pad. The addition of enzyme did not significantly impact meat quality traits (pH, cooking loss, water holding capacity, shear force and colour attributes). Chemical analysis showed significantly (p<0.05) higher Dry Matter (DM) and ash percent for breast meat and significantly (p<0.05) higher DM, ash and Crude Protein (CP) percent for thigh meat, in birds fed Con diet. In conclusion, enzyme supplementation elicited few responses in birds when supplemented at three levels in contrast to a normal corn-soybean diet.
References
Acamovic, T., 2001. Commercial application of enzyme technology for poultry production. Word's Poult. Sci. J., 57: 225-242.
AOAC, 2002. Official Methods of Analysis. 15th Edn., Association Official Analytical Chemistry, Arlington, VA.
Bedford, M.R., 2000. Exogenous enzymes in monogastric nutrition-their current value and future benefits. Anim. Feed Sci. Technol., 86: 1-13.
Bratcher, C.L., D.D. Johnson, R.C. Litell and B.L. Gwartney, 2005. The effects of quality grade, aging and location within muscle on Warner-Bratzler shear force in beef muscles of locomotion. Meat Sci., 70: 279-284.
Cafe, M.B., C.A. Borges, C.A. Fritts and P.W. Waldroup, 2002. Avizyme improves performance of broilers fed corn-soybean meal-based diets. J. Applied Poult. Res., 11: 29-33.
Costa, F.G.P., C.C. Goulart, D.F. Figueiredo, C.F.S. Oliveira and J.H.V. Silva, 2008. Economic and environmental impact of using exogenous enzymes on poultry feeding. Int. J. Poult. Sci., 7: 311-314.
Cowieson, A.J. and V. Ravindran, 2008. Effect of exogenous enzymes in maize-based diets varying in nutrient density for young broilers: Growth performance and digestibility of energy, minerals and amino acids. Br. Poult. Sci., 49: 37-44.
Cowieson, A.J. and V. Ravindran, 2008. Sensitivity of broiler starters to three doses of an enzyme cocktail in maize-based diets. Br. Poult. Sci., 49: 340-346.
Douglas, M.W., C.M. Parson and M.R. Bedford, 2000. Effect of various soybean meal sources and Avizyme on chick growth performance and ideal digestible energy. J. Applied Poult. Res., 9: 74-80.
El-Husseiny, O.M., A.G. Abdallah and K.O. Abdel-Latif, 2008. The influence of biological feed additives on broiler performance. Int. J. Poult. Sci., 7: 862-871.
Ghazi, S., J.A. Rooke and H. Galbraith, 2003. Improvement of the nutritive value of soybean meal by protease and α-galactosidase treatment in broiler cockerels and broiler chicks. Br. Poult. Sci., 44: 410-418.
Gilbert, C., T. Acamovic and M.R. Bedford, 2000. The effect of enzyme supplementation and lupin cultiveron chicks fed on lupin-based diets. Br. Poult. Sci., 41: 692-693.
Biswas, T., L. Mandal and S.K. Sarker, 1999. Studies of enzymes supplementation and herbal preparation at different levels of energy on the performance of broilers. J. Interacad., 3: 53-58.
Grau, R. and R. Hamm, 1953. A simple method for the determination of water binding in muscles. Naturwissennshaften, 40: 29-30.
Jeacocke, R.E., 1977. Continous measurement of the pH of beef muscle in intact beef carcasses. J. Food Technol., 12: 375-386.
Hughes, R.J., M. Choct, A. Kocher and R.J. van Barneveld, 2000. Effect of food enzymes on AME and composition of digesta from broiler chickens fed on diets containing non-starch polysaccharides isolated from lupin kernel. Br. Poult. Sci., 41: 318-323.
Jiang, Z., Y. Zhou, F. Lu, Z. Han and T. Wang, 2008. Effects of different levels of supplementary alpha-amylase on digestive enzyme activities and pancreatic amylase mRNA expression of young broilers. Asian-Aust. J. Anim. Sci., 21: 97-102.
Kidd, M.T., G.W. Morgan and C.J. Price, 2001. Enzyme supplementation to corn and soybean meal diets for broilers. J. Applied Poult. Res., 10: 65-70.
Kirk, R.S. and R. Sawyer, 1991. Pearson's Composition and Analysis of Foods. 9th Edn., Longman Singapore Publisher (Pte) Ltd., Singapore, pp: 430-450.
Kocher, A., M. Choct, M.D. Porter and J. Broz, 2002. Effects of feed enzymes on nutritive value of soybean meal fed to broilers. Br. Poult. Sci., 43: 54-63.
Kocher, A., M. Choct, G. Ross, J. Broz and T.K. Chung, 2003. Effects of enzyme combinations on apparent metabolizable energy of corn-soybean meal-based diets in broilers. J. Applied Poult. Res., 12: 275-283.
Manickam, R., K. Viswanathan and M. Mohan, 1994. Effect of probiotics in broiler performance. Ind. Vet. J., 71: 737-739.
Meng, X., B.A. Slominski, C.M. Nyachoti, C.M. Campbell and W. Guenter, 2005. Degredation of cell wall polysaccharides by combinations of carbohydrase enzymes and their effect on nutrient utilization and broiler chicken performance. Poult. Sci., 84: 37-47.
Mohamad, M.A. and A.S. Hamza, 1991. Using enzyme preparation in corn-soybean meal broiler rations. Egypt. J. Anim. Prod., 28: 245-254.
NRC., 1994. Nutrient Requirements of Poultry. 9th Edn., National Academy of Sciences, USA., pp: 19-34.
Odetallah, N.H., J.J. Wang, J.D. Garlich and J.C.H. Shih, 2003. Keratinase in starter diets improves growth of broiler chicks. Poult. Sci., 82: 664-670.
Ritz, C.W., R.M. Hulet, B.B. Self and D.M. Denbow, 1995. Growth and intestinal morphology of male turkeys as influenced by dietary supplementation of amylase and xylanase. Poult. Sci., 74: 1329-1334.
Safiudo, C., I. Sierra, M. lopez and F. Forcada, 1986. La qualita de la viande ovion. Etude des differents facteurs qui la conditionnent. Commission des C.E. Raport Eurebian, 11479: 67-81.
Saleh, F., A. Ohtsuka, T. Tanaka and K. Hayashi, 2004. Carbohydrases are digested by proteases present in enzyme preparations during in vitro digestion. J. Poult. Sci., 41: 229-235.
Saleh, F., M. Tahir, A. Ohtsuka and K. Hayashi, 2005. A mixture of pure cellulase, hemicellulase and pectinase improves broiler performance. Br. Poult. Sci., 46: 602-606.
Olukosi, O.A., A.J. Cowieson and O. Adeola, 2007. Age-related influence of a cocktail of xylanase, amylase and protease or phytase individually or in combination in broilers. Poult. Sci., 86: 77-86.
Olukosi, O.A., A.J. Coweison and O. Adeola, 2008. Influence of enzyme supplementation of maize-soybean meal diets on carcass composition, whole-body nutrient accretion and total tract nutrient retention of broilers. Poult. Sci., 49: 436-445.
Pack, M., M., Bedford and C. Watt, 1998. Feed enzymes may improve corn, sorghum diets. Feedstuufs, 2: 18-19.
Ranade, A.S. and B.V. Rajmane, 1992. Effect of enzyme feed supplement of commercial broilers. Proceedings of the 9th World Poultry Congress, Sept. 20-24, WPSA, Beekbeergen, Netherland, pp: 485-487.
Richter, G., A. Lemser and A. Muller, 1995. Is enzyme feeding beneficial in broiler feeding?: Lohnt sich der Einsatz von Enzymen in der Broilermast?. Muhie + Mischfutter Technik, 131: 546-547.
Samarasinghe, K., R. Messikommer and C. Wenk, 2000. Activity of supplementation enzymes and their effect on nutrient utilization and growth performance of growing chickens as affected by pelleting temperature. Arch. Anim. Nutr., 53: 45-58.
Sams, A.R. and D.M. Janky, 1986. The influence of brine chilling on tenderness of hot-boned, chill-boned and age-boned broiler breast fillets. Poult. Sci., 65: 1316-1321.
SAS, 2000. Propriety Software Release 8.1. Statistical Analysis Systems Institute Inc., Cary, North Carolina, USA.
Selle, P.H., D.G. Cadogan, Y.J. Ru and G.G. Partridge, 2010. Impact of exogenous enzymes in sorghum-or wheat-based broiler diets on nutrient utilization and growth performance. Int. J. Poult. Sci., 9: 53-58.
Vieira, S.L. R.P. Ott, J. Berres, A.R. Olmos, J.L.B. Coneglian and D.M. Freitas, 2006. Effects of a mix of carbohydrases on live performance and carcass yield of broilers fed all vegetable diets based on corn and soybean meal. Int. J. Poult. Sci., 5: 662-665.
Vranjes, M.V. and C. Wenk, 1995. Influence of dietary enzyme complex on the performance of broilers fed on diets with and without antibiotic supplementation. Br. Poult. Sci., 36: 265-275.
Wang, H., Y. Guo and J.C.H. Shih, 2008. Effects of dietary supplementation of keratinase on growth performance, nitrogen retention and intestinal morphology of broiler chickens fed diets with soybean meal and cottonseed meal. Anim. Feed Sci. Technol., 140: 376-384.
Werner, C., S. Janisch, U. Kumbet and M. Wicke, 2009. Comparative study of the quality of broiler and Turkey meat. Br. Poult. Sci., 50: 318-324.
Wyatt, C.L., E. Moran and M.R. Bedford, 1997. Utilizing feed enzymes to enhance the nutritional value of corn-based broiler diets. Poult. Sci., 76: 39-39.
Yamamoto, M., F. Saleh, M. Tahir, A. Ohtuska and K. Hayashi, 2007. The effect of Koji-feed (fermented distillery by-product) on the growth performance and nutrient metabolizability in broiler. J. Poult. Sci., 44: 291-296.
Zanella, I., N.K. Sakomura, F.G. Silversides, A. Fiqueirdo and M. Pack, 1999. Effect of enzyme supplementation of broiler diets based on corn and soybeans. Poult. Sci., 78: 561-568.
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