Detection of Group D Avian Rotaviruses among Layer Poultry from Western India
DOI:
https://doi.org/10.3923/ijps.2010.72.76Keywords:
Avian rotavirus, group D, RNA PAGE, RT-PCR, VP6Abstract
Rotavirus is a major cause of gastroenteritis in infants and children worldwide and also in wide variety of mammalian and avian species. In the present study, 4 (7.84%) out of the 54 poultry faecal samples tested, were found positive by RNA-PAGE. The samples were of 60-65 weeks age layer poultry birds suffering from diarrhoea. All the PAGE positive samples resolved in to 11 segments with a migration pattern of 5:2:2:2 characteristic of group D avian rotavirus. All the positive samples were of short electropherotype in which segments 10th and 11th migrated very closely. Avian rotavirus samples subjected to VP4 and VP7 gene based RT-PCR did not amplified respective products. RNA-PAGE positive samples were further subjected to VP6 gene based RT-PCR amplification using specific primers. All the four positive samples yielded a specific product of 493 bp. The study reports first ever detection of Group D avian rotavirus in Western India.
References
Ahmed, M.S. and M.U. Ahmed, 2006. Detection of avian rotavirus-like virus in broiler chickens in Bangladesh. Bangladesh J. Vet. Med., 4: 73-77.
Chomczynski, P. and N. Sacchi, 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Annu. Rev. Biochem., 162: 156-159.
Deswal, S., 2006. Genomic diversity analysis and nucleotide sequencing of hyper-variable regions in VP4 and VP7 genes of group A rotaviruses isolated from different host species. Dissertation. Faculty of Animal Biotechnology, Chaudhari Charan Singh Haryana Agricultural University. Hisar, India.
Estes, M.K. and J. Cohen, 1989. Rotavirus gene structure and function. Microbiol. Rev., 53: 410-449.
Estes, M.K., E.L. Palmer and J.F. Obijeski, 1983. Rotavirus: A review. Curr. Topics Microbiol., 105: 123-184.
Fedorova, O.F., N.A. Novikova, N.V. Epifanova, L.B. Lukovnikova, O.N. Kniagina, E.V. Gracheva and E.M. Bogacheva, 2005. Optimization of RT-PCR for the identification of VP4 gene of group A rotaviruses and evaluation of its diagnostic efficiency. Vopr Virusol., 50: 39-41.
Herring, A.J., N.F. Inglis, C.K. Ojeh, D.R. Snodgrass and J.D. Menzies, 1982. Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J. Clin. Microbiol., 16: 473-477.
Ito, H., N. Minamoto, I. Sasaki, H. Goto, M. Sugiyama, T. Kinjo and S. Sugita, 1995. Sequence analysis of cDNA for the VP6 protein of group a avian rotavirus: A comparison with group a mammalian rotaviruses. Arch. Virol., 140: 605-612.
Kalica, A.R., R.G. Wyatt and A.Z. Kapikian, 1978. Detection of differences among human and animal rotaviruses using analysis of viral RNA. J. Am. Vet. Med. Assoc., 173: 531-537.
Kang, G., M. Iturriza-Gomara, J.G. Wheeler, P. Crystal and B. Monica et al., 2004. Quantitation of group A rotavirus by real-time reverse-transcription-polymerase chain reaction: Correlation with clinical severity in children in South India. J. Med. Virol., 73: 118-122.
Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.
Legrottaglie, R., V. Rizzi and P. Agrimi, 1997. Isolation and identification of avian rotavirus from pheasant chicks with signs of clinical enteritis. Comp. Immunol. Microb., 20: 205-210.
McNulty, M.S., G.M. Allan and J.C. Stuart, 1978. Rotavirus infection in avian species. Vet. Rec., 103: 319-320.
Mcnulty, M.S., G. Mallan, D. Todd, J.B. Mcferran and R.M. Mccrcken, 1981. Isolation from chickens of a rotavirus lacking the rotavirus group antigen. J. Gen. Virol., 55: 405-413.
Mcnulty, M.S., G.M. Alla and J.B. Mcferran, 1984. Prevalence of antibody to conventional and atypical rotaviruses in chickens. Vet. Rec., 114: 219-219.
Mcnulty, M.S., G.M. Allan, D. Todd, B. Mcfarran, E.R. Mckillop, D.S. Colins and R.M. Mccracken, 1980. Isolation of rotaviruses from turkey and chicken, demonstration of distinct serotypes and RNA electropherotypes. Avian Pathol., 9: 363-375.
Minakshi, P., G. Prasad, S. Verma and S. Dahiya, 2004. Detection of group a avian rotaviruses from diarrhoeic poultry in India. In. J. Microbiol., 44: 205-209.
Saif, L.J., 1989. Immunodiagnosis of enteric viral infections application of electron microscopy and monoclonal antibodies. Proceedings of 38th Western Poultry Disease Conference, March 6-9, Tempe, Ariz, pp: 232-237.
Savita, A.L. Kusumakar, Y.P.S. Malik, P. Minakshi and G. Prasad, 2008. Detection and characterization of group A and D avian rotaviruses in India. Indian J. Biotechnol., 7: 554-556.
Svensson, L., I. Uhnoo, M. Grandien and G. Wadell, 1986. Molecular epidemiology of rotavirus infections in Uppsala, Sweden, 1981: Disappearance of a predominant electropherotype. J. Med. Virol., 18: 101-111.
Tamechiro, C.Y., A.F. Alfieri, K.C. Medici and A.A. Alfieri, 2003. Segmented double-stranded genomic RNA viruses in faecal samples from broiler chicken. Braz. J. Microbiol., 34: 349-353.
Todd, D. and M.S. McNulty, 1986. Electrophoretic variation of avian rotavirus RNA in polyacrylamide gels. Avian Pathol., 15: 149-159.
Wani, S.A., M.A. Bhat, S.M. Ishaq, M.A. Ashrafi, A.S. Buchh and M. Haq, 2003. Detection of a mammalian-like group A rotavirus in diarrhoeic chicken. Vet. Microbiol., 94: 13-18.
Yashon, C.V. and K.A. Schat, 1985. Isolation and characterization of avian rotaviruses. Avian Dis., 29: 499-508.
Bergeland, M.E., J.P. Mcadaragh and I. Stotz, 1997. Rotaviral enteritis in Turkey poults. Proceedings of the 26th Western Poultry Disease Conference, (WPDC'97), Davis, Ca., pp: 129-130.
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