Enterococcus Species Isolated from Northern Bobwhite (Colinus virginianus) Chicks: Species Distribution and Antibiotic Resistance
DOI:
https://doi.org/10.3923/ijps.2010.43.47Keywords:
Enterococcus, multiple antibiotic resistance, northern bobwhiteAbstract
Intestinal fragments from newly hatched to seven-day-old Northern Bobwhite chicks (n = 39) were processed to isolate organisms belonging to the genus Enterococcus. Identification of the eighty-nine enterococci revealed forty-two E. saccharolyticus, twenty E. malodoratus, eight E. pseudoavium, seven E. raffinosus, six E. faecalis, two E. mundtii, two E. avium, one E. cecorum and one E. durans. Each isolate was examined for its resistance to ampicillin, ciprofloxacin, clindamycin, erythromycin, nitrofurantoin, tetracycline, and vancomycin by the disk diffusion assay. Our results indicate all isolates were resistant to at least two of the antibiotics examined, sixty-nine were resistant to three different antibiotics, while sixteen isolates were resistant to four of the seven antibiotics examined. Most significantly, eighteen enterococcal isolates, five of which were E. faecalis, were shown to be vancomycin resistant and possess a minimal inhibitory concentration of >160 μg/ml. In many of the isolates, the genetic location of the antibiotic resistance genes was shown to be present on conjugative plasmids.
References
Aarestrup, F.M., Y. Agerso, P.Gerner-Smith, M. Madsen and L.B. Jensen, 2000. Comparison of antimicrobial resistance phenotypes and resistance genes in Enterococcus faecalis and Enterococcus faecium from humans in the community, broilers and pigs in Denmark. Diagnostic Microbiol. Infect. Dis., 37: 127-137.
Arthur, M. and P. Courvalin, 1993. Genetics and mechanisms of glycopeptide resistance in enterococci. Antimicrob. Agents Chemother., 37: 1563-1571.
Anonymous, 1997. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved Standard. M7-A4/National Committee for Clinical Laboratory Standards (NCCLS), Wayne, PA.
Anonymous, 1998. Development of in vitro susceptibility testing criteria and quality control parameters. NCCLS document M23-T2. National Committee for Clinical Laboratory Standards, Wayne, PA.
Anonymous, 1998. Performance standards for antimicrobial disk susceptibility tests. Approved Standard M2-A6. National Committee for Clinical Laboratory Standards, Wayne, PA.
Anonymous, 1999. Performance standards for antimicrobial susceptibility testing. Supplement M100-S9, National Committee for Clinical Laboratory Standards, Wayne, PA.
Baele, M., L.A. Devriese, P. Butaye and F. Haesebrouck, 2002. Composition of enterococcal and streptococcal flora from pigeon intestines. J. Appl. Micro., 92: 348-351.
Blaimont, B., J. Charlier and G. Wauters, 1995. Comparative distribution of Enterococcus species in faecaes and clinical samples. Microb. Ecol. Health Dis., 8: 87-92.
Borgen, K., G.S. Simonsen, A. Sundsfjord, Y. Wasteson, O. Olsvik and H. Kruse, 2000. Continuing high prevalence of VanA-type vancomycin-resistant enterococci on Norwegian poultry farms three years after avoparcin was banned. J. Appl. Microbiol., 89: 478-485.
Bryce, E.A., S.J.V. Zemcov and A.M. Clarke, 1991. Species identification and antibiotic resistance patterns of the enterococci. Eur. J. Clin. Microb. Infect. Dis., 10: 745-747.
Bustamante, W., A. Alpizar, S. Hernandez, A. Pacheco and N. Vargas et al., 2003. Predominance of vanA genotype among vancomycin-resistant Enterococcus isolates from poultry and swine in Costa Rica. Appl. Environ. Microb., 69: 7414-7419.
Devriese, L.A., J.I.C. Cloque, P. Deherdt and F. Haesebrouck, 1992. Identification and composition of the tonsilar and anal enterococci and streptococcal flora of dogs and cats. J. Appl. Bacteriol., 73: 421-425.
Devriese, L.A., L. Laurier, P. Deherdt and F. Haesebrouck, 1992. Enterococcal and streptococcal species isolated from faeces of calves, young cattle and dairy cows. J. Applied Bacteriol., 72: 29-31.
Dutka, B.J. and K.K. Kawn, 1978. Comparison of eight media-procedures for recovering faecal streptococci from water under winter conditions. J. Applied Bacteriol., 45: 333-340.
Facklam, R.R. and M.D. Collins, 1989. Identification of Enterococcus species isolated from human infections by a conventional test scheme. J. Clin. Microbiol., 27: 731-734.
Facklam, R.R., D.F. Sahm and L.M. Teixeira, 1999. Enterococcus. In: Manual of Clinical Microbiology, Murray, P.R., E.J. Baron, M.A. Pfaller, F.C. Tenover and R.H. Yolken (Eds.). 7th Edn., American Society for Microbiology Press, Washington, DC., pp: 297-305.
Guiney, M. and G. Urwin, 1993. Frequency and antimicrobial susceptibility of clinical isolates of enterococci. Eur. J. Clin. Microb. Infect. Dis., 12: 362-366.
Huys, G., K. D'Haene, J.M. Collard and J. Swings, 2004. Prevalence and molecular characterization of tetracycline resistance in Enterococcus isolates from food. Applied Environ. Microl., 70: 1555-1562.
Joseph, S.W., J.R. Hayes, L.L. English, L.E. Carr and D.D. Wagner, 2001. Implications of multiple antimicrobial-resistant enterococci associated with the poultry environment. Food Additives Contam., 18: 1118-1123.
Kibby, H.J., C. Hagedorn and E.L. McCoy, 1978. Use of fecal streptococci as indicators of pollution in soil. Applied Environ. Micro., 35: 711-717.
Klare, I., H. Heier, H. Claus, R. Reissbrodt and W. Witte, 1995. VanA-mediated high-level glycopeptide resistance in Enterococcus faecium from animal husbandry. FEMS Microb. Lett., 125: 165-171.
Laukova, A., M. Marounek and K. Bod'a, 1995. Characteristics of enterococci and staphylococci isolated from the crop and caecum of Japanese quails exposed to microgravity conditions. Vet. Med., 40: 317-321.
Murray, B.E., 1991. New aspects of antimicrobial resistance and the resulting therapeutic dilemmas. J. Infect. Dis., 163: 1184-1194.
Rice, E.W., J.W. Messer, C.H. Johnson and D.J. Reasoner, 1995. Occurrence of high-level aminoglycoside resistance in environmental isolates of enterococci. Applied Environ. Microl., 61: 374-376.
Spera, R.V. and B.F. Farber, 1994. Multidrug-resistant Enterococcus faecium. An untreatable noscomial pathogen. Drugs, 48: 678-688.
Thal, L.A., J.W. Chow, R. Mahayni, H. Bonilla and M.B. Perri et al., 1995. Characterization of antimicrobial resistance in enterococci of animal origin. Antimicrob. Agents Chemother., 39: 2112-2115.
Teuber, M., V. Perreten and F. Wirsching, 1996. Antibiotikumresistente bakterien: Eine neue dimension in der lebensmittelmikrobiologie. Lebensmittel-Technol., 29: 182-199.
Welton, L.A., L.A. Thal, M.B. Perri, S. Donabedian, J. Mcmahon, J.W. Chow and M.J. Zervos, 1998. Antimicrobial resistance in enterococci isolated from Turkey flocks fed virginiamycin. Antimicrob. Agents Chemother., 42: 705-708.
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