Precipitation of Clinical Infections in Chickens by Infectious Bursal Disease Virus Preserved under Different Storage Temperatures
DOI:
https://doi.org/10.3923/ijps.2009.1058.1061Keywords:
Infectious bursal disease, infectivity, storageAbstract
Biological activities and precipitation of clinical infection potentials of Infectious Bursal Disease Virus (IBDV) stored under different storage conditions were assessed in cockerel chicks. Infective bursae were stored for between 10-20 months at different storage temperature (+4oC, -20oC and -196oC), homogenized and used to challenge IBD-antibody-naïve chickens at 21 days of age. Clinical infections were precipitated in varying degrees. The sample stored at -196oC produced the most marked effect (signs, symptoms and deaths) in susceptible chickens and had an index score of 8.700; followed by the bursae tissue stored at -20oC with an index score of 5.167. The tissue stored at +4oC produced barely insignificant symptomatic/clinical effects in the chickens. This affirms that biological activities of infectious organisms like IBDV is best maintained at deep freezing or ultra low temperature.
References
Durojaiye, O.A., H.A. Ajibade and G.O. Olafimihan, 1984. An outbreak of infectious bursal disease in 20 weeks old birds. Trop. Vet., 2: 175-176.
Faragher, J.T., W.H. Allan and C.J. Wyeth, 1974. Immunosuppressive effect of infectious bursal agent on vaccination against Newcastle disease. Vet. Rec., 95: 385-388.
Lukert, P.D. and Y.M. Saif, 2003. Infectious Bursal Disease. In: Diseases of Poultry, Saif, Y.M., H.J. Barnes and J.R. Glisson (Eds.). 11th Edn., Blackwell Scientific Publisher, Ames, Iowa, pp: 161-179.
Makadiya, N.R., 2004. Detection of infectious bursal disease virus (IBDV) from bursal tissue by RT-PCR and its comparative efficacy with conventional precipitation assays. M.Sc. Thesis, Anand Agricultural University
Nwosuh, C.I., P.A. Okewole, E.P. Aba-Adulugba and P.S. Odeyemi, 1989. Outbreak of infectious bursal disease in a flock in Kano. Trop. Vet., 7: 32-34.
Okoye, J.O.A. and M. Uzuokwu, 1982. Characterization of Nigerian strain of infectious bursal disease virus of chicken: Clinico-pathological manifestation of naturally occurring field outbreaks. Bull. Anim. Health Prod. Afr., 30: 193-197.
Onunkwo, O., 1975. An outbreak of infectious bursal disease of chicken in Nigeria. Vet. Rec., 97: 433-433.
OIE. (World Organisation for Animal Health), 2008. Infectious bursal disease (Gumboro disease). http://www.oie.int/eng/normes/mmanual/2008/pdf/2.03.12_IBD.pdf.
OIE. (World Organisation for Animal Health), 2008. Newcastle Disease. In: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Epizooties, O.I.D. (Ed.). Office of International des Epizooties, Paris, France, pp: 567-589.
Downloads
Published
Issue
Section
License
Copyright (c) 2009 Asian Network for Scientific Information

This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.