Assessment of Pathogenic Potential of Two Indian H5N1 Highly Pathogenic Avian Influenza Virus Isolates by Intravenous Pathogenicity Index Test
DOI:
https://doi.org/10.3923/ijps.2009.283.290Keywords:
HPAI, India, ivpi, pathology, rt-pcrAbstract
Intravenous Pathogenicity Index (IVPI) test was conducted to assess the pathogenicity of two H5N1 (A/Ck/Ind/7966/06 and A/Ck/Ind/7972/06) AIV isolates. Both the H5N1 virus isolates were isolated from natural outbreaks during 2006, both isolates caused death of all birds with in 48 h after inoculation experimentally, birds showed typical clinical signs, gross and microscopic lesions of Avian influenza. IVPI of A/Ck/Ind/7966/06 and A/Ck/Ind/7972/06 was 2.96 and 2.95 respectively. This test showed that both H5N1 isolates are highly pathogenic. Virus could be re-isolated from all the organs of infected chickens and it was reconfirmed by RT-PCR using WHO and Lee primers.
References
Bankowski, R.A., 1982. Introduction and objectives of the symposium. Proceedings of the 1st International Symposium on Avian Influenza, (ISAI'82), Richmond, VA., pp: 6-14.
Claas, E.C.J., A.D.M. Osterhaus, R. van Beek, J. de Jong and G.F. Rimmelzwaan et al., 1998. Human influenza A H5N1 virus related to a highly pathogenic avian influenza virus. Lancet, 351: 472-477.
Lamb, R., 1989. Genes and Proteins of the Influenza Viruses. In: The Influenza Viruses, Krug, R.M. (Ed.). Plenum Press, New York, pp: 1-67.
Lee, M.S., P.C. Chang, J.H. Shien, M.C. Cheng and S.K. Shieh, 2001. Identification and subtyping of avian influenza viruses by reverse transcription PCR. J. Virol. Methods, 97: 13-22.
Lee, C.W., D.E. Swayne, A.J. Linares, D.A. Senne and D.L. Suarez, 2005. H5N2 Avian influenza outbreak in texas in 2004: The first highly pathogenic strain in the united states in 20 years? J. Virol., 79: 11412-11421.
Nguyen, D.C., T.M. Uyeki, S. Jadhao, T. Maines, S. Michel and D.E. Swayne et al., 2005. Isolation and characterization of avian influenza viruses including H5N1, from live bird markets in Hanoi, Vitnam in 2001. J. Virol., 79: 4201-4212.
Office International Epizootics, 2005. Evolution of the animal health situation with regard to avian influenza. Aug. 29, 2005.
Swayne, D.E., 1997. Pathobiology of H5N2 mexican ai virus infections of chickens. Vet. Pathol., 34: 557-567.
Tumpey, T.M., D.L. Suarez, L.E.L. Perkins, D.A. Senne and J. Lee et al., 2003. Evaluation of a high-pathogenicity H5N1 avian influenza A virus isolated from duck meat. Avian Dis., 47: 951-955.
WHO., 2002. WHO Manual on Animal Influenza Diagnosis and Surveillance. World Health Organization, Geneva.
Wood, G.W., J.W. McCauley, J.B. Bashiruddin and D.J. Alexander, 1993. Deduced amino acid sequences at the haemagglutinin cleavage site of avian influenza A viruses of H5 and H7 subtypes. Arch. Virol., 130: 209-217.
Yilmaz, F., N. Timurkaan and F. Coven, 2004. Pathological findings in quails infected with avian influenza A virus H7 n1 subtype. Int. J. Poult. Sci., 3: 764-767.
Yuen, K.Y., P.K.S. Chan, M. Peiris, D.N.C. Tsang and T.L. Que et al., 1998. Clinical features and rapid viral diagnosis of human disease associated with avian influenza A H5N1 virus. Lancet, 351: 467-471.
Downloads
Published
Issue
Section
License
Copyright (c) 2009 Asian Network for Scientific Information
This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
