Diagnosis of Mycoplasma gallisepticum from a Broiler Breeder Flock: Comparison of Three Diagnostics Methods


Authors

  • J.D. Evans USDA-Agricultural Research Service, Poultry Research Unit, Mississippi State, MS 39762, USA
  • D.L. Thornton Mississippi Board of Animal Health, Carthage, MS 39051, Mississippi State University,Mississippi State, MS 39762, USA
  • S.L. Branton USDA-Agricultural Research Service, Poultry Research Unit, Mississippi State, MS 39762, USA

DOI:

https://doi.org/10.3923/ijps.2009.104.107

Keywords:

Broiler breeders, chronic respiratory disease, diagnostics, Mycoplasma gallisepticum, NPIP, poultry

Abstract

NPIP-mandated serological screening of a multiplier breeder flock detected possible Mycoplasma gallisepticum (MG) exposure. The flock was quarantined and further samples including blood and choanal swabs were collected and sent to a research facility for independent testing and confirmation. Subsequent analyses included diagnosis by Serum Plate Agglutination (SPA), MG-specific Polymerase Chain Reaction (PCR) and culture identification. Results of the various diagnostic tests were compared. The findings of the various diagnostic tests were in agreement and confirmed MG infection of the breeder flock. Time requirements of the various diagnostic procedures were recorded and were 1 h, 27 h and 30 days for SPA, MG-specific PCR and culture identification, respectively. The results affirm the validity of the diagnostic procedures and emphasize the importance of timely screening and diagnostic procedures for control of MG.

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Published

2009-01-15

Issue

Vol. 8 No. 2 (2009)

Section

Research Article

License

Copyright (c) 2009 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Evans, J., Thornton, D., & Branton, S. (2009). Diagnosis of Mycoplasma gallisepticum from a Broiler Breeder Flock: Comparison of Three Diagnostics Methods. International Journal of Poultry Science, 8(2), 104–107. https://doi.org/10.3923/ijps.2009.104.107