Construction of Genomic DNA Library of Beijing Fatty Chick In lemda phage EMBL3 and Screen of Chick Heart Type Fatty Acid Binding Protein Gene


Authors

  • M.H. Ye National Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 10094, PR China College of Bio-Science and Biotechnology, Yangzhou University, Yangzhou, 225009 PR China
  • J. Wen National Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 10094, PR China
  • H.H. Cao National Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 10094, PR China
  • H.B. Li National Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 10094, PR China
  • J.L. Chen National Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 10094, PR China
  • G.P. Zhao National Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 10094, PR China
  • M.Q. Zheng National Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 10094, PR China

DOI:

https://doi.org/10.3923/ijps.2007.599.603

Keywords:

Beijing fatty chick, DNA library, fatty acid binding protein gene, screen

Abstract

A genomic DNA library of Beijing Fatty chick was constructed in phage EMBL3 with a titer of 3.85×105. To testify the utility of this library we screened recombinant phages containing chick heart type fatty acid binding protein gene by Southern hybridization. In order to simplify the process of screen we determined sub-library which contained target gene firstly by PCR with specific primers. Several positive recombinants were detected. We selected one randomly to be digested with Sal I. Results showed inserted exogenous DNA was about 15Kb.

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Published

2007-07-15

Issue

Vol. 6 No. 8 (2007)

Section

Research Article

License

Copyright (c) 2007 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Ye, M., Wen, J., Cao, H., Li, H., Chen, J., Zhao, G., & Zheng, M. (2007). Construction of Genomic DNA Library of Beijing Fatty Chick In lemda phage EMBL3 and Screen of Chick Heart Type Fatty Acid Binding Protein Gene. International Journal of Poultry Science, 6(8), 599–603. https://doi.org/10.3923/ijps.2007.599.603