Monitoring the Effect of Disinfection Methods on Mycoplasma gallisepticum in Commercial Layer Farms
DOI:
https://doi.org/10.3923/ijps.2022.28.37Keywords:
<i>Mycoplasma gallisepticum</i>, commercial poultry farm, egg-layer flocks, mortality, production performanceAbstract
Background and Objective: It is important for poultry owners to control disinfectants resistance of Mycoplasma gallisepticum circulating amongst poultry farms. On a field level among poultry flocks a survey study was conducted to identify and to estimate the effects of the most common poultry pathogens (Mycoplasma gallisepticum) on production performance of commercial layer hens and the efficacy of the most common disinfectants against Mycoplasma gallisepticum was determined through traditional and new methods of application against MG strain isolated from commercial layer farms in Egypt and recorded in Gen-Bank. Materials and Methods: A survey study was carried out to identify and to estimate the effects of Mycoplasma gallisepticum on production performance of 15 commercial layer flocks. In addition, the efficacy of some commercially available disinfectants against Mycoplasma gallisepticum with different application methods was also monitored. Results: The results showed that: (1) The prevalence of M. gallisepticum (33.3%) and mortality rate (12%) was high in the flocks at 78 weeks of age and the current egg-production performance was also significantly reduced (p<0.05). (2) MG isolate accessed on Gen-Bank and coded as; MZ826700 , 26 bp DNA linear BCT 30-SEP-2021, DEFINITION Mycoplasma gallisepticum strain EGY2021 mgc2 gene, partial cds. ACCESSION MZ826700-authers: Alfeteehy, N.M., Mohamed, M. and Kaoud, H.A. (3) Fogging method showed the highest reduction in Mycoplasma gallisepticum populations. Conclusion: Mycoplasma gallisepticum infection leads to tremendous economic losses in poultry production as a result of decreased hatchability, egg production and mortalities; it is evident that, fogging will increase the efficacy of the used disinfectants for 15 min contact of exposure time.
References
Levisohn, S. and S.H. Kleven, 2000. Avian mycoplasmosis (Mycoplasma gallisepticum). Rev. Sci. Tech., 19: 425-442.
Armour, N.K. and N. Ferguson-Noel, 2015. Evaluation of the egg transmission and pathogenicity of Mycoplasma gallisepticum isolates genotyped as ts-11. Avian Pathol., 44: 296-304.
Vance, A.M., S.L. Branton, S.D. Collier, P.D. Gerard and E.D. Peebles, 2008. Effects of prelay ts11-strain Mycoplasma gallisepticum inoculation and time-specific F-strain M. gallisepticum inoculation overlays on internal egg and eggshell characteristics of commercial laying hens. Poult. Sci., 87: 1358-1363.
Feberwee, A., D.R. Mekkes, D. Klinkenberg, J.C.M. Vernooij, A.L.J. Gielkens and J.A. Stegeman, 2005. An experimental model to quantify horizontal transmission of Mycoplasma gallisepticum. Avian Pathol., 34: 355-361.
Bradbury, J.M., 2001. Avian Mycoplasmosis. In: Poultry Diseases. Jordan, F., W.B. Saunders Company, Jordan, pp: 178-193.
Branton, S.L., S.M.D. Bearson, B. Bearson, B.D. Lott and W.R. Maslin et al., 2002. The effects of 6/85 live Mycoplasma gallisepticum vaccine in commercial layer hens over a 43-week laying cycle on egg production, selected egg quality parameters and egg size distribution when challenged before beginning of lay. Avian Dis., 46: 423-428.
Raviv, Z. and D.H. Ley, 2013. Mycoplasma gallisepticum Infection. In: Diseases of Poultry, Swayne, D.E., J.R. Glisson, L.R. McDougald, L.K. Nolan, D.L. Suarez and V.L. Nair, (Eds.). Wiley-Blackwell, Hoboken, New Jersey, Pages: 1408.
Valks, M. and D.G. Burch, 2002. Comparative activity and resistance development of tiamulin and other antimicrobials against Avian mycoplasma. Presentation at the World Veterinary Poultry Association.
Ley, D.H., 2003. Mycoplasma gallisepticum infection. In: Diseases of Poultry, Saif, Y.M., H.J. Barnes, A.M. Fadly, J.R. Glisson, L.R. McDougald and D.E. Swayne (Eds.). Iowa State Press, Ames, Iowa, pp: 722-744.
Marois, C., F. Dufour-Gesbert and I. Kempf, 2002. Polymerase chain reaction for detection of Mycoplasma gallisepticum in environmental samples. Avian Pathol., 31: 163-168.
Feberwee, A., D.R. Mekkes, J.J. de Wit, E.G. Hartman and A. Pijpers, 2005. Comparison of culture, PCR and different serologic tests for detection of Mycoplasma gallisepticum and Mycoplasma synoviae infections. Avian Dis., 49: 260-268.
Landman, W.J.M. and A. Feberwee, 2004. Aerosol-induced Mycoplasma synoviae arthritis: The synergistic effect of infectious bronchitis virus infection. Avian Pathol., 33: 591-598.
Adell, E., S. Calvet, A. Pérez-Bonilla, A. Jiménez-Belenguer, J. García, J. Herrera and M. Cambra-López, 2015. Air disinfection in laying hen houses: Effect on airborne microorganisms with focus on Mycoplasma gallisepticum. Biosyst. Eng., 129: 315-323.
Peebles, E.D., E.Y. Basenko, S.L. Branton, S.K. Whitmarsh, D.V. Maurice and P.D. Gerard, 2006. Effects of S6-strain Mycoplasma gallisepticum inoculation at ten, twenty-two, or forty-five weeks of age on the egg yolk composition of commercial egg-laying hens. Poult. Sci., 85: 1502-1508.
Khalda, K.A., 2002. Characterization of Glucose Positive Mycoplasma in with Respect to Mycoplasma gallisepticum in Sudan. Master Thesis, University of Khartoum.
Khalifa, K.A., E.S. Abdelrahim, M. Badwi and A.M. Mohamed, 2013. Isolation and molecular characterization of Mycoplasma gallisepticum and Mycoplasma synoviae in chickens in Sudan. J. Vet. Med., Vol. 2013.
García, M., N. Ikuta, S. Levisohn and S.H. Kleven, 2005. Evaluation and comparison of various PCR methods for detection of Mycoplasma gallisepticum infection in chickens. Avian Dis., 49: 125-132.
Lysnyansky, I., M. Garcia and S. Levisohn, 2005. Use of mgc2-polymerase chain reaction-restriction fragment length polymorphism for rapid differentiation between field isolates and vaccine strains of Mycoplasma gallisepticum in Israel. Avian Dis., 49: 238-245.
Office International des Epizooties OIE., 2008. Manual of standards for Diagnostic Tests and Vaccines. Office International des Epizooties O.I.E., Paris.
Kleven, S.H., 2008. Control of avian Mycoplasma infections in commercial poultry. Avian Dis., 52: 367-374.
Payne, J.B., E.C. Kroger and S.E. Watkins, 2005. Evaluation of disinfectant efficacy when applied to the floor of poultry grow-out facilities. J. Applied Poult. Res., 14: 322-329.
Raviv, Z. and S.H. Kleven, 2009. The development of diagnostic real-time TaqMan PCRs for the four pathogenic avian mycoplasmas. Avian Dis., 53: 103-107.
Nikfarjam, L. and P. Farzaneh, 2012. Prevention and detection of Mycoplasma contamination in cell culture. J. Cell., 13: 203-212.
Islam, K.M.S., U. Klein and D.G.S. Burch, 2009. The activity and compatibility of the antibiotic tiamulin with other drugs in poultry medicine-A review. Poult. Sci., 88: 2353-2359.
Feberwee, A., T.S. de Vries and W.J.M. Landman, 2008. Seroprevalence of Mycoplasma synoviae in dutch commercial poultry farms. Avian. Pathol., 37: 629-633.
Nagatomo, H., Y. Takegahara, T. Sonoda, A. Yamaguchi, R. Uemura, S. Hagiwara and M. Sueyoshi, 2001. Comparative studies of the persistence of animal mycoplasmas under different environmental conditions. Vet. Microbiol., 82: 223-232.
Eterpi, M., G. McDonnell and V. Thomas, 2011. Decontamination efficacy against Mycoplasma. Lett. Applied Microbiol., 52: 150-155.
Dunowska, M., P.S. Morley and D.R. Hyatt, 2005. The effect of Virkon®s fogging on survival of Salmonella enterica and Staphylococcus aureus on surfaces in a veterinary teaching hospital. Vet. Microbiol., 105: 281-289.
Vardar, C., K. Ilhan and O.A. Karabulut, 2012. The application of various disinfectants by fogging for decreasing postharvest diseases of strawberry. Postharvest Biol. Technol., 66: 30-34.
Barua, H., P.K. Biswas, K.E.P. Olsen, S.K. Shil and J.P. Christensen, 2013. Molecular characterization of motile serovars of Salmonella enterica from breeder and commercial broiler poultry farms in Bangladesh. PLOS ONE, Vol. 8.
Rimi, N.A., R. Sultana, M. Muhsina, B. Uddin and N. Haider et al., 2017. Biosecurity conditions in small commercial chicken farms, Bangladesh 2011–2012. EcoHealth, 14: 244-258.
Stringfellow, K., P. Anderson, D. Caldwell, J. Lee and J. Byrd et al., 2009. Evaluation of disinfectants commonly used by the commercial poultry industry under simulated field conditions. Poult. Sci., 88: 1151-1155.
Islam, K.N., K.N. Monira, R. Sultana and I.M. Azharul, 2007. The effect of Timsen and Ambicide as disinfectant on hatchability traits of Kasila broiler parents eggs in Bangladesh. Livest. Res. Rural Dev., Vol. 19.
McDonnell, G. and A.D. Russell, 1999. Antiseptics and disinfectants: Activity, action and resistance. Clin. Microbiol. Rev., 12: 147-179.
Saklou, N.T., B.A. Burgess, D.C.V. Metre, K.J. Hornig, P.S. Morley and S.R. Byers, 2015. Comparison of disinfectant efficacy when using high-volume directed mist application of accelerated hydrogen peroxide and peroxymonosulfate disinfectants in a large animal hospital. Equine Vet. J., 48: 485-489.
Sher, M. and R. Mulder, 2020. Comparison of aerosolized hydrogen peroxide fogging with a conventional disinfection product for a dental surgery. J. Contemp. Dent. Pract., 21: 1307-1311.
Ahmed, R. and R. Mulder, 2021. A systematic review on the efficacy of vaporized hydrogen peroxide as a non-contact decontamination system for pathogens associated with the dental environment. Int. J. Environ. Res. Public Health, Vol. 8.
McAuliffe, L., R.J. Ellis, K. Miles, R.D. Ayling and R.A.J. Nicholas, 2006. Biofilm formation by mycoplasma species and its role in environmental persistence and survival. Microbiology, 152: 913-922.
Davies, R. and M. Breslin, 2003. Observations on Salmonella contamination of commercial laying farms before and after cleaning and disinfection. Vet. Record, 152: 283-287.
Ruano, M., J. El-Attrache and Pedrovillegas, 2001. Efficacy comparisons of disinfectants used by the commercial poultry industry. Avian Dis., 45: 972-977.
Singh, S., R.K. Agarwal, S.C. Tiwari and H. Singh, 2011. Antibiotic resistance pattern among the Salmonella isolated from human, animal and meat in India. Trop. Anim. Health Prod., 44: 665-674.
Justice-Allen, A., J. Trujillo, R. Corbett, R. Harding, G. Goodell and D. Wilson, 2010. Survival and replication of Mycoplasma species in recycled bedding sand and association with mastitis on dairy farms in Utah. J. Dairy Sci., 93: 192-202.
Finnegan, M., E. Linley, S.P. Denyer, G. McDonnell, C. Simons and J.Y. Maillard, 2010. Mode of action of hydrogen peroxide and other oxidizing agents: Differences between liquid and gas forms. J. Antimicrob. Chemother., 65: 2108-2115.
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