Prolactin Hormone Profile, Patterns and Expression Level of Prolactin, Pit-1, VIP and PREB Gene in Kampung Chicken (Gallus gallus domesticus) Induced by Anti-Prolactin
DOI:
https://doi.org/10.3923/ijps.2021.249.255Keywords:
Kampung chicken, Pit-1 gene, PREB gene, Prl gene, prolactin hormone profile, VIP geneAbstract
Background and Objective: Prolactin is a steroid hormone that induces broodiness in chickens. In addition to the prolactin hormone, the incubating nature of chickens is also influenced by genes. The purpose of this study was to analyze changes in the profile of the prolactin hormone; the pattern and level of expression of the genes Prolactin (Prl), Vasoactive Intestine Peptide (VIP), Pituary specific transcription factor-1 (Pit-1) and prolactin regulatory element binding protein (PREB) due to the administration of each dose of antiprolactin (2-Bromo-α-Ergocryptine Methanesulfonate salt/Bromocriptine); as well as the relationship between prolactin hormone secretion and these genes. Materials and Methods: Blood and brain samples from 25 hens (Kampung chickens) fed various doses of anti-prolactin were used in this study. Serum from each blood sample was used to calculate the concentration of the hormone prolactin by ELISA method. Meanwhile, the brains of each free-range chicken were used to measure the pattern and level of expression of the Prl, VIP, Pit-1 and PREB genes using the Quantitative Real Time-Polymerase Chain Reaction (RT-qPCR) method. Results: Increase in the doses of anti-prolactin decreased the concentration of the hormone prolactin as well as the pattern and level of expression of the Prl, VIP, Pit-1 and PREB genes. Conclusion: Anti-prolactin can inhibit the secretion of the hormone prolactin and the expression of Prl, Pit-1, VIP and PREB genes.
References
Marano, R.J. and N. Ben-Jonathan, 2014. Minireview: Extrapituitary prolactin: An update on the distribution, regulation, and functions. Mol. Endocrinol., 28: 622-633.
Riddle, O., R.W. Bates and E.L. Lahr, 1935. Prolactin induces broodiness in fowl. Am. J. Physiol., 111: 352-360.
Romanov, M.N., 2001. Genetics of broodiness in poultry - A review. Asian-Australas. J. Anim. Sci., 14: 1647-1654.
Elsholtz, H.P., A.M. Lew, P.R. Albert and V.C. Sundmark, 1991. Inhibitory control of prolactin and pit-1 gene promoters by dopamine. Dual signaling pathways required for D2 receptor-regulated expression of the prolactin gene. J. Bio. Chem., 266: 22919-22925.
Sartika, T., 2005. Broodiness trait of chicken through molecular investigation. Wartazoa: Indonesian Bull. Anim. Vet. Sci., 15: 206-212.
Shen, X., H. Zeng, L. Xie, J. He and J. Li et al., 2012. The GTPase activating Rap/RanGPA domain-like 1 gene is associated with chicken reproductive traits. PLoS ONE, Vol. 7.
Hiyama, G., N. Kansaku, T. Tanaka, S. Wakui and D. Zadworny, 2015. Characterization of chicken prolactin regulatory element binding protein and its expression in the anterior pituitary gland during embryogenesis and different reproductive stages. J. Poult. Sci., 52: 42-51.
Hiyama, G., N. Kansaku, S. Wakui, R. McQuaid and D. Zadworny, 2015. Characterization and expression of Turkey prolactin regulatory element binding in the anterior pituitary gland and pancreas during embryogenesis. J. Poult. Sci., 53: 67-75.
Sweetman, S.C., 2009. Martindale: The Complete Drug Reference. 36th Edn., Pharmaceutical Press, London, Uk.
Bacharach, A.L. and D.R. Laurence, 1964. Preface. In: Evaluation of Drug Activities: Pharmacometrics Laurence, D.R. and A.L. Bacharach, (Eds.). Academic Press, New York, Pages: 4.
Buntin, J.D., R.M. Hnasko and P.H. Zuzick, 1999. Role of the ventromedial hypothalamus in prolactin-induced hyperphagia in ring doves. Physiol. Behav., 66: 255-261.
Sharp, P.J., A. Dawson and R.W. Lea, 1998. Control of luteinizing hormone and prolactin secretion in birds. Comp. Biochem. Physiol. Part C: Pharmacol., Toxicol. Endocrinol., 119: 275-282.
Berry, W., 2003. The physiology of induced molting. Poult. Sci., 82: 971-980.
Molik, E. and M. Błasiak, 2015. The role of melatonin and bromocriptine in the regulation of prolactin secretion in animals - A review. Ann. Anim. Sci., 15: 849-860.
Freeman, M.E., B. Kanyicska, A. Lerant and G. Nagy, 2000. Prolactin: Structure, function, and regulation of secretion. Physiol. Rev., 80: 1523-1631.
Kansaku, N., G. Hiyama, T. Sasanami and D. Zadworny, 2008. Prolactin and growth hormone in birds: Protein structure, gene structure and genetic variation. J. Poult. Sci., 45: 1-6.
Youngren, O.M., Y. Chaiseha and M.E.E. Halawani, 1998. Regulation of prolactin secretion by dopamine and vasoactive intestinal peptide at the level of the pituitary in the Turkey. Neuroendocrinology, 68: 319-325.
Usman, M., A. Basheer, M. Akram, M.E. Babar and I. Zahoor, 2014. Prolactin: Candidate gene for egg production or broodiness traits in chicken. Sci. Int., 26: 1191-1195.
Talbot, R.T., M.C. Hanks, R.J. Sterling, H.M. Sang and P.J. Sharp, 1991. Pituitary prolactin messenger ribonucleic acid levels in incubating and laying hens: effects of manipulating plasma levels of vasoactive intestinal polypeptide. Endocrinology, 129: 496-502.
Kansaku, N., K. Shimada, N. Saito and H. Hidaka, 1998. Effects of protein kinase A inhibitor (H-89) on VIP-and GRF-induced release and mRNA expression of prolactin and growth hormone in the chicken pituitary gland. Comp. Biochem. Physiol. C: Pharmacol. Toxicol. Endocrinol., 119: 89-95.
Kang, S.W., O.M. Youngren and M.E.E. Halawani, 2002. Influence of VIP on prolactinemia in Turkey anterior pituitary cells: Role of cAMP second messenger in VIP-induced prolactin gene expression. Regul. Peptides, 109: 39-44.
Guemene, D. N. Kansaku and D. Zadworny, 2001. Incubation behaviour expression in Turkey hens and its control in commercial flocks. Prod. Anim., 14: 147-160.
Ottinger, M.A. and M.R. Bakst, 1995. Endocrinology of the avian reproductive system. J. Avian Med. Surg., 9: 242-250.
Xu, H., X. Shen, M. Zhou, M. Fang, H. Zeng, Q. Nie and X. Zhang, 2010. The genetic effects of the dopamine D1 receptor gene on chicken egg production and broodiness traits. BMC Genet., Vol. 11.
Kahtane, A.A., Y. Chaiseha and M.E. Halawani, 2003. Dopaminergic regulation of avian prolactin gene transcription. J. Mol. Endocrinol., 31: 185-196.
Fliss, M.S., P.M. Hinkle and C. Bancroft, 1999. Expression cloning and characterization of PREB (prolactin regulatory element binding), a novel WD motif DNA-binding protein with a capacity to regulate prolactin promoter activity. Mol. Endocrinol., 13: 644-657.
Fitzgerald, P. and T.G. Dinan, 2008. Prolactin and dopamine: What is the connection? A review. J. Psychopharmacol., 22: 12-19.
Lincoln, G.A., H. Andersson and D. Hazlerigg, 2003. Clock genes and the long-term regulation of prolactin secretion: Evidence for a photoperiod/circannual timer in the pars tuberalis. J. Neuroendocrinol., 15: 390-397.
Bjøro, T., O. Sand, B.C. Østberg, J.O. Gordeladze, P. Torjesen, K.M. Gautvik and E. Haug, 1990. The mechanisms by which vasoactive intestinal peptide (VIP) and thyrotropin releasing hormone (TRH) stimulate prolactin release from pituitary cells. Biosci. Rep., 10: 189-199.
Hurley, J.H., 1999. Structure, mechanism, and regulation of mammalian adenylyl cyclase. J. Bio. Chem., 274: 7599-7602.
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