Effects of a Plant Based Natural Alternative of Choline and Betaine as Replacements for Synthetic Choline in Broilers Diets
DOI:
https://doi.org/10.3923/ijps.2001.24.30Keywords:
Betaine, broiler, choline chloride, growth performance, natural cholineAbstract
Objective: This study aimed to assess the feasibility of replacing synthetic Choline Chloride (CC) with a natural Choline Alternative (NC) and betaine (BT) in broiler diets, focusing on their effects on broiler performance. Materials and Methods: Ten dietary treatments were tested on 3,000 male Ross-308 broiler chicks over a 42-day period, including varying levels of Natural Choline (NC), betaine (BT) and synthetic Choline Chloride (CC), with 10 replicates per treatment. Birds were housed in 100 pens, with each pen serving as an experimental unit. Results: The study found a significant effect of diet on BW at 42 days. Broilers fed 400 g/ton of either BT or NC exhibited higher BW compared to the control, birds fed with 200 g/ton of NC and broilers fed with CC. The most efficient Feed Conversion Ratio (FCR) was observed in broilers fed the combination of NC 100 g/ton and BT 200 g/ton, followed by those fed BT or NC at 200 g/ton each. No significant differences (p>0.05) were observed among these treatments. Broilers supplemented with CC and the control group showed the least efficient FCR. The combination of NC and BT showed better performance. Conclusion: These findings suggest that NC and BT can replace synthetic CC in broiler diets without compromising performance. Moreover, the combination of NC and BT may provide a synergistic benefit, potentially leading to better feed efficiency and growth performance. This indicates that natural choline alternatives can be a viable replacement for synthetic choline chloride in broiler nutrition.
References
Calderano, A.A., R.V. Nunes, R.J.B. Rodrigueiro and R.A. César, 2015. Replacement of choline chloride by a vegetal source of choline in diets for broilers. Cienc. Anim. Bras., 16: 37-44.
Lin, C.W., T.W. Huang, Y.J. Peng, Y.Y. Lin, H.J. Mersmann and S.T. Ding, 2020. A novel chicken model of fatty liver disease induced by high cholesterol and low choline diets. Poult. Sci., Vol. 100.
Corbin, K.D. and S.H. Zeisel, 2011. Choline metabolism provides novel insights into nonalcoholic fatty liver disease and its progression. Curr. Opin. Gastroenterol., 28: 159-165.
Boesze-Battaglia, K. and R.J. Schimmel, 2021. Cell membrane lipid composition and distribution: implications for cell function and lessons learned from photoreceptors and platelets. J. Exp. Biol., 200: 2927-2936.
Wauben, I.P.M. and P.E. Wainwright, 2010. The influence of neonatal nutrition on behavioral development: A critical appraisal. Nutr. Rev., 57: 35-44.
Igwe, I., C. Okonkwo, U. Uzoukwu and C. Onyenegecha, 2015. The effect of choline chloride on the performance of broiler chickens. Annu. Res. Rev. Biol., Vol. 8, No. 3.
NRC., 1994. Nutrient Requirements of Poultry. 9th Edn., National Academy Press, Washington, DC., USA., ISBN-13: 9780309048927, Pages: 176.
Zhang, C., M. Pan, B. Li, L. Wang and X. Mo et al., 2012. Choline and betaine intake is inversely associated with breast cancer risk: A two‐stage case‐control study in China. Cancer Sci., 104: 250-258.
Khosravi, S., J.W. Jang, S. Rahimnejad, J.W. Song and K.J. Lee, 2015. Choline essentiality and its requirement in diets for juvenile parrot fish (oplegnathus fasciatus). Asian-Australas. J. Anim. Sci., 28: 647-653.
Lugata, J.K., A.D.S.V. Ortega and C. Szabó, 2022. The role of methionine supplementation on oxidative stress and antioxidant status of poultry-A review. Agriculture, Vol. 12.
Gül, E.T., O. Olgun, G. Kılınç, A. Yıldız and A. Sarmiento-García, 2023. Does the addition of choline and/or betaine to diets reduce the methionine requirements of laying quails? Assessment of performance and egg antioxidant capacity. Poult. Sci., Vol. 102.
Baker, D.H. and M. Sugahara, 2013. Nutritional investigation of the metabolism of glycine and its precursors by chicks fed a crystalline amino acid diet. Poult. Sci., 49: 756-760.
Selvam, R., M. Saravanakumar, S. Suresh, C.V. Chandrasekeran and D. Prashanth, 2018. Evaluation of polyherbal formulation and synthetic choline chloride on choline deficiency model in broilers: implications on zootechnical parameters, serum biochemistry and liver histopathology. Asian-Australas. J. Anim. Sci., 31: 1795-1806.
Zeisel, S.H., K.A. daCosta, M. Youssef and S. Hensey, 2018. Conversion of dietary choline to trimethylamine and dimethylamine in rats: dose-response relationship. J. Nutr., 119: 800-804.
Khose, K., S. Manwar, M. Gole, R. Ingole and P. Rathod, 2018. Efficacy of herbal choline as a replacement of synthetic choline chloride in diets on growth performance of broilers. Int. J. Livest. Res., Vol. 8.
White, D., W. Kim and S. Maini, 2019. Role of biocholine on growth performance, hepatic gene expression, and adiponectin in broilers. Proceedings of the International Poultry Scientific Forum, February 1-12, 2019, Georgia World Congress Center, 1-14.
Petrolli, T.G., R.R. Petrolli, E. Aniecevski, C.S. Facchi and F. Leite et al., 2021. Vegetable choline as a replacement for choline chloride in broiler feed. Acta Sci. Anim. Sci., Vol. 43.
Suárez-Suárez, N.E., H.A. Lee-Rangel, A.C. Lizarazo-Chaparro, G.D. Mendoza-Martínez and G. Espinosa-Reyes et al., 2023. Effect of the supplementation using an herbal mixture as a choline source during early gestation in Rambouillet ewes. Animals, Vol. 13.
Farina, G., A. de Mello Kessler, P.D. Ebling, F.R. Marx, R. César and A.M.L. Ribeiro, 2017. Performance of broilers fed different dietary choline sources and levels. Ciênc. Anim. Bras., Vol. 18.
Aviagen, 2022. Ross broiler: Nutrient specifications – All plant protein-based feeds. https://aviagen.com/assets/Tech_Center/Ross_Broiler/Ross-PlantProteinBasedBroilerNutritionSpecifications2022-EN.pdf
Jadhav, N.V., V. Nagbhushana, S. Maini and S.M. Kartikesh, 2008. An evaluation of comparative effects of feeding synthetic and herbal choline on broiler performance, nutrient balance and serum activities. Vet. World, 1: 306-309.
PESTI, G.M., N.J. BENEVENGA, A.E. HARPER and M.L. SUNDE, 2012. Factors influencing the assessment of the availability of choline in feedstuffs. Poult. Sci., 60: 188-196.
Molitoris, B.A. and D.H. Baker, 2018. Choline utilization in the chick as influenced by levels of dietary protein and methionine. J. Nutr., 106: 412-418.
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