Comparative Evaluation of Haemagglutination Inhibition Test and Enzyme-linked Immunosorbent Assay for Detection of Antibodies Against Newcastle Disease Vaccine in Broiler Chicks


Authors

  • M.H. Tabidi Department of Animal Production, College of Agricultural Studies, Sudan University of Science and Technology, Khartoum North, Sham bat, P.O. Box 71, Sudan
  • A. Makkawi Department of Animal Production, College of Agricultural Studies, Sudan University of Science and Technology, Khartoum North, Sham bat, P.O. Box 71, Sudan
  • A.S. Ali Department of Preventive Medicine, Faculty of Veterinary Medicine, University of Khartoum, Khartoum North, P.O. Box 32, Sudan

DOI:

https://doi.org/10.3923/ijps.2004.668.670

Keywords:

Antibody, ELISA, HI, ND

Abstract

The antibody (Ab) titres to the intermediate Newcastle disease virus (NDV) vaccine (Komorov strain) in broiler chicks using haemagglutination inhibition (HI) test and an indirect enzyme-linked immunosorbent assay (ELISA) were compared in this study. The tirtres were compared following vaccination of chicks via the aerosol, intranasal and drinking water routes. For all routes of the vaccine administration, higher Ab tritres were detected using ELISA technique than HI test. For both serological assays, the highest Ab titres detected when the vaccine was administered via the aerosol route with significant level (p< 0.05) compared to the control group. Non-consistent pattern in the Ab levels between the two tests was observed for intranasal and drinking water routes. As a conclusion, ELISA proved more accurate, sensitive and rapid but less economic than HI test when used for detection of Ab titres against NDV vaccines.

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Published

2004-09-15

Issue

Vol. 3 No. 10 (2004)

Section

Research Article

License

Copyright (c) 2004 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Tabidi, M., Makkawi, A., & Ali, A. (2004). Comparative Evaluation of Haemagglutination Inhibition Test and Enzyme-linked Immunosorbent Assay for Detection of Antibodies Against Newcastle Disease Vaccine in Broiler Chicks. International Journal of Poultry Science, 3(10), 668–670. https://doi.org/10.3923/ijps.2004.668.670