The Effect of Different Extenders on Some Fertility Properties of Roosters Semen
DOI:
https://doi.org/10.3923/ijps.2019.504.507Keywords:
Mass activity, milk extender, rooster sperm, semen motility, semen viabilityAbstract
Background and Objective: Poultry sperm gradually loses its quality within an hour of sample collection. This study aimed to determine the storage effects of different extenders (at 4°C) on rooster sperm fertility properties. Materials and Methods: Eighteen seminal fluid samples of a Lohman Brown strain were collected by dorsal-abdominal massage. These were divided into three groups and added to three extenders: a simple medium for assisted reproductive technology (SMART), Tris and milk. Since non-diluted fresh sperm loses its quality within an hour of semen collection, no control group was used. Percentages of mass activity, motility, normality and viability were tested. These were done at 1, 4 and 8 h after semen collection. Completely Random Design (C.R.D) was used in this experiment. Results: At the first timepoint, the semen test score of the SMART medium extender was higher than that of the other extenders. In contrast, the milk extender shows a highly significant improvement in sperm parameters post preservation at both the second and the third time tests. The SMART extender was protecting sperm vigor more than Tris at most of the tested timepoints. Conclusion: Extenders delay the loss of rooster sperm fertilization ability. Full milk extender was better than other extenders in the protection of sperm fertility.
References
Donoghue, A.M. and G.J. Wishart, 2000. Storage of poultry semen. Anim. Reprod. Sci., 62: 213-232.
Alkan, S., A. Baran, Ö.B. ÖzdaŞ and M. Evecen, 2002. Morphological defects in Turkey semen. Turk. J. Vet. Anim. Sci., 26: 1087-1092.
Foote, R.H., 2002. Within-herd use of boar semen at 5°C, with a note on electronic monitoring of oestrus. Reprod. Domest. Anim., 37: 61-63.
Sexton, T.J. and T.A. Fewlass, 1978. A new poultry semen extender: 2. Effect of the diluent components on the fertilizing capacity of chicken semen stored at 5°C. Poult. Sci., 57: 277-284.
Ngoula, F., T.T. Tebug, A. Kenfack, F.H. Defang, F. Tendonkeng and T.E. Pamo, 2012. Effects of buck age, storage duration, storage temperature and diluent on fresh west african dwarf buck semen. J. Reprod. Infertil., 3: 58-66.
Habibullah, M., M.A. Hashem, M.S. Rana and M.H. Islam, 2015. Effect of artificial insemination on different production parameter in hubbard classic broiler parent stock. J. Bangladesh Agric. Univ., 13: 71-77.
Brillard, J.P., 1993. Sperm storage and transport following natural mating and artificial insemination. Poult. Sci., 72: 923-928.
Hudson, G.H., A.V. Omprakash and K. Premavalli, 2016. Effect of semen diluents and dilution rates on motility of guinea fowl spermatozoa under short-term storage. Indian Vet. J., 93: 13-15.
Parker, H.M. and C.D. McDaniel, 2003. Semen dilution prior to analysis influences the ability of the sperm quality analyzer to predict fertility whether inseminating with a constant number of sperm or a constant volume of semen. Poult. Sci., 82: 1808-1815.
Abouelezz, F.M.K., C. Castano, A. Toledano-Diaz, M.C. Esteso, A. Lopez-Sebastian, J.L. Campo and J. Santiago-Moreno, 2015. Sperm-egg penetration assay assessment of the contraceptive effects of glycerol and egg yolk in rooster sperm diluents. Theriogenology, 83: 1541-1547.
Kuzlu, M. and A. Taskin, 2017. The effect of different extenders on the sperm motility and viability of frozen turkey semen. Indian J. Anim. Res., 51: 235-241.
Rahman, M.S., M.R. Gofur, M.M. Rahman, F.Y. Bari and N.S. Juyena, 2018. Effect of skim milk and tris-citrate extenders to preserve the semen of indigenous ram of Bangladesh. Asian J. Biol., 5: 1-11.
Al-Daraji, H.J., 2012. Effect of diluent supplementation with different levels of orange juice on semen quality during liquid storage of Roosters' semen. Int. J. Vet. Sci., 1: 5-9.
El-Nasry, E., H.M. Khalil, M. Abaza and A. El-Saadany, 2004. Use of antioxidants in storing local cockerels semen. 1. Effects on semen quality and fertility. Proceedings of the 22nd World's Poultry Congress, June 8-13, 2004, Istanbul, Turkey, pp: 231-235.
Fakhrildin, M.B.M.R. and N.K. Flayyih, 2011. A new simple medium for in vitro sperm activation of asthenozoospermic patients using direct swim-up technique. Kufa Med. J., 14: 67-75.
Evans, G. and W.M.C. Maxwell, 1987. Salamon's Artificial Insemination of Sheep and Goats. Butterworths Pvt. Ltd., Sydney, Australia, ISBN-13: 9780409491777, Pages 196.
Karabinus, D.S., D.P. Evenson and M.T. Kaproth, 1991. Effects of egg yolk-citrate and milk extenders on chromatin structure and viability of cryopreserved bull sperm. J. Dairy Sci., 74: 3836-3848.
Blom, E. and N.O. Christensen, 1947. Studies on the pathological conditions in the testis, epididymis and accessory glands in the bull. Skand. Vet. Tidsskr, 37: 1-49.
Chemineau, P., Y. Guerin, Y. Caginie, P. Arguer and J.C. Vallet, 1991. Training manual on artificial insemination in sheep and goats. FAO Animal Production and Health Paper No. 83, FAO, Rome, Italy.
Hancock, J.L., 1952. The morphology of bull spermatozoa. J. Exp. Biol., 29: 445-453.
Swanson, E.W. and H.J. Bearden, 1951. An eosin-nigrosin stain for differentiating live and dead bovine spermatozoa. J. Anim. Sci., 10: 981-987.
Sikka, S.C., 2004. Role of oxidative stress and antioxidants in andrology and assisted reproductive technology. J. Androl., 25: 5-18.
Al-Taji, H.A., 1994. Sperm activation and intrauterine insemination: The effect of sperm concentration and culture media on sperm activation potential in vitro. M.Sc. Thesis, College of Medicine, University of Baghdad, Iraq.
Parks, J.E. and D.V. Lynch, 1992. Lipid composition and thermotropic phase behavior of boar, bull, stallion and rooster sperm membranes. Cryobiology, 29: 255-266.
Pillai, B.K., R. Jasuja, J.R. Simard and J.A. Hamilton, 2009. Fast diffusion of very long chain saturated fatty acids across a bilayer membrane and their rapid extraction by cyclodextrins: Implications for adrenoleukodystrophy. J. Biol. Chem., 284: 33296-33304.
Brunaldi, K., N. Huang and J.A. Hamilton, 2010. Fatty acids are rapidly delivered to and extracted from membranes by methyl-β-cyclodextrin. J. Lipid Res., 51: 120-131.
Bergeron, A., Y. Brindle, P. Blondin and P. Manjunath, 2007. Milk caseins decrease the binding of the major bovine seminal plasma proteins to sperm and prevent lipid loss from the sperm membrane during sperm storage. Biol. Reprod., 77: 120-126.
Khaeim, H.M., 2013. Mass selection with an optical sorter for head scab resistance in soft red winter wheat. Master's Thesis, College of Agriculture, Food and Environment, University of Kentucky, Lexington, KY., USA.
Downloads
Published
Issue
Section
License
Copyright (c) 2019 The Author(s)
This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
