The Development of Multiplex PCR Microsatellite Marker Sets for Korean Chicken Breeds


Authors

  • Sung Soo Choi Genomic Informatics Center, Hankyong National University, Anseong 17579, South Korea
  • Joo Hee Seo Genomic Informatics Center, Hankyong National University, Anseong 17579, South Korea
  • Jung-Min Han Genomic Informatics Center, Hankyong National University, Anseong 17579, South Korea
  • Jiyeon Seong Genomic Informatics Center, Hankyong National University, Anseong 17579, South Korea
  • Jun Heon Lee Division of Animal & Dairy Science, Chungnam National University, Daejeon 34134, South Korea
  • Hong Sik Kong Genomic Informatics Center, Hankyong National University, Anseong 17579, South Korea

DOI:

https://doi.org/10.3923/ijps.2019.492.498

Keywords:

Breeding, chicken, hanhyup, Korean native chicken, microsatellite, multiplex PCR marker, polymorphisms

Abstract

Background and Objective: Korea’s National Institute of Animal Science (NIAS) has preserved two types of purebred chicken strains and the Hanhyup Company has maintained several purebred chicken stains. This study aimed to facilitate the analyses of genetic diversity, parentage and population structure through the isolation and characterization of novel multiplex PCR microsatellite (MS) marker sets for Korean chicken breeds. Materials and Methods: Genotyping of all 27 markers was performed for 469 purebred chicken samples from 20 Korean breeds. Of these, 16 highly polymorphic MS markers were selected based on the number of alleles, expected heterozygosity (Hexp) and polymorphic information content (PIC). The selected markers were classified into two sets of 16 markers each. Results: The expected probability of identity values for the 16 MS markers in random individuals (PI), random half-sib (PIhalf-sibs) and random sibs (PIsibs) were estimated at 1.79×10–21, 6.76×10–16 and 5.70×10–8 respectively. Conclusion: Our multiplex PCR marker sets were observed to have favorable applications in the conservation, planning of breeding and traceability systems in the Korean chicken breeds. Additionally, the marker sets will be of considerable use in small- and large-scale population genetic structure analyses.

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Published

2019-09-15

Issue

Vol. 18 No. 10 (2019)

Section

Research Article

License

Copyright (c) 2019 The Author(s)

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This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Choi, S. S., Seo, J. H., Han, J.-M., Seong, J., Lee, J. H., & Kong , H. S. (2019). The Development of Multiplex PCR Microsatellite Marker Sets for Korean Chicken Breeds. International Journal of Poultry Science, 18(10), 492–498. https://doi.org/10.3923/ijps.2019.492.498