Effect of Digestrom® and Poultry Star® on the Body Performance and Immunity Status of Broiler Chickens
DOI:
https://doi.org/10.3923/ijps.2018.385.391Keywords:
Digestrom®, ELISA, growth performance, Newcastle disease, poultry diet, Poultry Star®Abstract
Objective: This study was carried out to evaluate the positive effects of two different commercial products (Digestrom® and Poultry Star®) on body performance and the immune response against Newcastle disease after challenge with a virulent local strain in broiler chickens routinely vaccinated against Newcastle disease. Materials and Methods: A total of 250 one-day-old broiler chicks were randomly distributed to five groups (G1-G5) with 50 chicks per group and treated as follows, G1: Received Digestrom® as a feed additive at a dose of 150 mg kg–1, G2: Received Poultry Star® as a feed additive at a dose of 0.5 g kg–1 (2×1011 CFU kg–1), G3: Received Digestrom® at a dose of 150 mg kg–1 and Poultry Star® at a dose of 0.5 g kg–1 (2×1011 CFU kg–1) as feed additives, G4: Positive control (vaccinated but not treated), G5: Negative control (vaccinated and treated). All groups except G5 (negative control) were vaccinated with NDV LaSota and 14-days-old IBDV D78 intermediate at 5, 10 and 20 days of age via drinking water. At the age of 25 days, all groups were challenged with the virulent strain of Newcastle disease at 100 ELD50 106. Results: The current study showed the highest variable antibody titer (Abs) in the third group that was fed Digestrom® and Poultry Star® with significant increases (p<0.05) in serum total protein, albumin and globulin levels, average daily feed intake and average daily gain (p<0.05) as well as a significant decrease (p<0.05) in morbidity and mortality, followed by the other groups (G1 and G2) that were fed with one product, compared to the positive control group (G4). Meanwhile, the results were significantly lower in the negative control group (G5) than in all other groups. The results following challenge with virulent NDV were very high in G5 and very low in G3. Conclusion: Dietary inclusion of 150 ppm Digestrom® improved body performance, which led to increased serum total protein levels. Moreover, both individual and combined application of Digestrom® and Poultry Star® increased the immune response against NDV, with synergistic effects observed with combined treatment.
References
Dorman, H.J.D. and S.G. Deans, 2000. Antimicrobial agents from plants: Antibacterial activity of plant volatile oils. J. Appl. Microbiol., 88: 308-316.
Ultee, A., E.P.W. Kets and E.J. Smid, 1999. Mechanisms of action of carvacrol on the food-borne pathogen Bacillus cereus. Applied Environ. Microbiol., 65: 4606-4610.
Alcicek, A., M. Bozkurt and M. Cabuk, 2004. The effect of a mixture of herbal essential oils, an organic acid or a probiotic on broiler performance. S. Afr. J. Anim. Sci., 34: 217-222.
Zhang, K.Y., F. Yan, C.A. Keen and P.W. Waldroup, 2005. Evaluation of microencapsulated essential oils and organic acids in diets for broiler chickens. Int. J. Poult. Sci., 4: 612-619.
Zohair, G.A.M., 2006. Recent prophylactic and control aspect of certain chicken bacterial problems. Ph.D. Thesis, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.
Jamroz, D., J. Orda, C. Kamel, A. Wiliczkiewicz, T. Wertelecki and J. Skorupinska, 2003. The influence of phytogenic extracts on performance, nutrient digestibility, carcass characteristics and gut microbial status in broiler chickens. J. Anim. Feed Sci., 12: 583-596.
Radwan, M.A., G.A. Abd-Allah, H.M. Fayek and M.A. Breiweek, 1995. The effect of three types of feed additives on the productive performance of layers. Proceedings of the 1st Egyptian Hungarian Conference, (EHC'95), Alexandria, Egypt.
Guo, X., D. Li, W. Lu, X. Piao and X. Chen, 2006. Screening of Bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs. Antonie Leeuwenhoek, 90: 136-146.
Talebi, A., B. Amirzadeh, B. Mokhtari and H. Gahri, 2008. Effects of a multi-strain probiotic (PrimaLac) on performance and antibody responses to newcastle disease virus and infectious bursal disease virus vaccination in broiler chickens. Avian Pathol., 37: 509-512.
Acamovic, T. and J.D. Brooker, 2005. Biochemistry of plant secondary metabolites and their effects in animals. Proc. Nutr. Soc., 64: 403-412.
Hashemipour, H., H. Kermanshahi, A. Golian and T. Veldkamp, 2013. Effect of thymol and carvacrol feed supplementation on performance, antioxidant enzyme activities, fatty acid composition, digestive enzyme activities and immune response in broiler chickens. Poult. Sci., 92: 2059-2069.
Khattak, F., A. Ronchi, P. Castelli and N. Sparks, 2014. Effects of natural blend of essential oil on growth performance, blood biochemistry, cecal morphology, and carcass quality of broiler chickens. Poult. Sci., 93: 132-137.
Synbiotic® Corporation, 2005. Newcastle disease virus antibody test kit. Proflock R. Plus, Item No. 96-95 33, Frontera, San Diego, CA., USA.
Langhout, P., 2000. New additives for broiler chickens. World Poult., 16: 22-27.
Reed, L.J. and H. Muench, 1938. A simple method of estimating fifty percent endpoints. Am. J. Epidemiol., 27: 493-497.
SAS., 2012. SAS User's Guide: Statistics. Version 9.1, SAS Institute Inc., Cary, NC., USA.
Alp, M., M. Midilli, N. Kocabagli, H. Yilmaz, N. Turan, A. Gargili and N. Acar, 2012. The effects of dietary oregano essential oil on live performance, carcass yield, serum immunoglobulin G level and oocyst count in broilers. J. Applied Poult. Res., 21: 630-636.
Hong, J.C., T. Steiner, A. Aufy and T.F. Lien, 2012. Effects of supplemental essential oil on growth performance, lipid metabolites and immunity, intestinal characteristics, microbiota and carcass traits in broilers. Livestock Sci., 144: 253-262.
Luna, A., M.C. Labaque, J.A. Zygadlo and R.H. Marin, 2010. Effects of thymol and carvacrol feed supplementation on lipid oxidation in broiler meat. Poult. Sci., 89: 366-370.
Ahmad, I., 2006. Effect of probiotics on broilers performance. Int. J. Poult. Sci., 5: 593-597.
Ng, S.C., A.L. Hart, M.A. Kamm, A.J. Stagg and S.C. Knight, 2009. Mechanisms of action of probiotics: Recent advances. Inflamm. Bowel Dis., 15: 300-310.
Rahimi, S. and A. Khaksefidi, 2006. A comparison between the effect of a probiotic (Bioplus 2B) and an antibiotic (virginiamycin) on the performance of broiler chickens under heat stress condition. Iranian J. Vet. Res., 7: 23-28.
Zhang, Y., L. Ren, W. Li, Q. Xu, G. Chang, X. Wang and B. Li, 2012. Cloning of neuraminidase (NA) gene and identification of its antiviral activity. Afr. J. Biotechnol., 11: 10675-10681.
Havenaar, R. and S. Spanhaak, 1994. Probiotics from an immunological point of view. Curr. Opin. Biotechnol., 5: 320-325.
Lee, K.W., H. Everts and A.C. Beynen, 2004. Essential oils in broiler nutrition. Int. J. Poult. Sci., 3: 738-752.
Lee, K.W., H. Everts, H.J. Kappert, M. Frehner, R. Losa and A.C. Beynen, 2003. Effects of dietary essential oil components on growth performance, digestive enzymes and lipid metabolism in female broiler chickens. Br. Poult. Sci., 44: 450-457.
Amad, A.A., K. Manner, K.R. Wendler, K. Neumann and J. Zentek, 2011. Effects of a phytogenic feed additive on growth performance and ileal nutrient digestibility in broiler chickens. Poult. Sci., 90: 2811-2816.
Botsoglou, N.A., P. Florou-Paneri, E. Christaki, D.J. Fletouris and A.B. Spais, 2002. Effect of dietary oregano essential oil on performance of chickens and on iron-induced lipid oxidation of breast, thigh and abdominal fat tissues. Br. Poult. Sci., 43: 223-230.
Celikbilek, A., G. Deniz, A. Orman, H. Gencoglu and C. Kara, 2014. Effects of a combination of dietary organic acid blend and oregano essential oil (Lunacompacid® Herbex dry) on the performance and Clostridium perfringens proliferation in the ileum of broiler chickens. J. Biol. Environ. Sci., 8: 61-69.
Crawford, J.S., 1979. Probiotics in animal nutrition. Proceedings of the Arkansas Nutrition Conference, September 27-28, 1979, Arkansas, USA., pp: 45-55.
Mohan, B., R. Kadirvel, A. Natarajan and M. Bhaskaran, 1996. Effect of probiotic supplementation on growth, nitrogen utilisation and serum cholesterol in broilers. Br. Poult. Sci., 37: 395-401.
Mead, G.C., 1989. Microbes of the avian cecum: Types present and substrates utilized. J. Exp. Zool., 252: 48-54.
Jin, L.Z., Y.W. Ho, N. Abdullah and S. Jalaludin, 1997. Probiotics in poultry: Modes of action. World's Poult. Sci. J., 53: 351-368.
Ahmad, M., M. Chaudhry, M.F. Rai and H.B. Rashid, 2007. Evaluation of two vaccination schemes using live vaccines against Newcastle disease in chickens. Turk. J. Vet. Anim. Sci., 31: 165-169.
Fioramonti, J., V. Theodorou and L. Bueno, 2003. Probiotics: What are they? What are their effects on gut physiology? Best Pract. Res. Clin. Gastroenterol., 17: 711-724.
Williams, P. and R. Losa, 2001. The use of essential oils and their compounds in poultry nutrition. World Poult., 17: 14-15.
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