In vitro Antibiotic Susceptibility Patterns of Ornithobacterium rhinotracheale from Commercial Chickens in Argentina#


Authors

  • Carlos D. Gornatti Churria Catedra de Patología de Aves y Pilíferos y Laboratorio de Diagnóstico de Enfermedades de las Aves y los Pilíferos, Facultad de Ciencias Veterinarias, 60 y 118 s/n, CC 296 B1900AVW, Universidad Nacional de La Plata, La Plata, Buenos Aires Province, Argentina
  • Panayiotis Loukopoulos Catedra de Patología de Aves y Pilíferos y Laboratorio de Diagnóstico de Enfermedades de las Aves y los Pilíferos, Facultad de Ciencias Veterinarias, 60 y 118 s/n, CC 296 B1900AVW, Universidad Nacional de La Plata, La Plata, Buenos Aires Province, Argentina
  • German B. Vigo Veterinary Diagnostic Laboratory, Building 295, School of Animal and Veterinary Sciences, Charles Sturt University, Nathan Cobb Drive, Wagga Wagga, New South Wales 2550 and Graham Centre for Agricultural Innovation, Charles Sturt University, Locked Bag 588, Wagga Wagga, New South Wales 2678, Australia
  • Pablo Sansalone Phibro Vaccines, Phibro Animal Health Argentina SRL, Av. Alicia M. de Justo N° 1848 1° Piso Oficina 16, 1107 AFL, Ciudad Autónoma de Buenos Aires, Buenos Aires Province, Argentina
  • Mariana A. Machuca Cátedra de Patología Especial y Laboratorio de Patología Especial Veterinaria "Dr. B. Epstein", Facultad de Ciencias Veterinarias, 60 y 118 s/n, CC 296 B1900AVW, Universidad Nacional de La Plata, La Plata, Buenos Aires Province, Argentina
  • Victorio Nievas Catedra de Microbiología y Laboratorio de Diagnostico e Investigaciones Bacteriologicas, Facultad de Ciencias Veterinarias, 60 y 118 s/n, CC 296 B1900AVW, Universidad Nacional de La Plata, La Plata, Buenos Aires Province, Argentina
  • Miguel Piscopo Catedra de Patología de Aves y Pilíferos y Laboratorio de Diagnóstico de Enfermedades de las Aves y los Pilíferos, Facultad de Ciencias Veterinarias, 60 y 118 s/n, CC 296 B1900AVW, Universidad Nacional de La Plata, La Plata, Buenos Aires Province, Argentina
  • Miguel Herrero Loyola Catedra de Patología de Aves y Pilíferos y Laboratorio de Diagnóstico de Enfermedades de las Aves y los Pilíferos, Facultad de Ciencias Veterinarias, 60 y 118 s/n, CC 296 B1900AVW, Universidad Nacional de La Plata, La Plata, Buenos Aires Province, Argentina
  • Miguel A. Petruccelli Catedra de Patología de Aves y Pilíferos y Laboratorio de Diagnóstico de Enfermedades de las Aves y los Pilíferos, Facultad de Ciencias Veterinarias, 60 y 118 s/n, CC 296 B1900AVW, Universidad Nacional de La Plata, La Plata, Buenos Aires Province, Argentina

DOI:

https://doi.org/10.3923/ijps.2016.293.296

Keywords:

Antibioticsusceptibilities, commercialchickens, hemolyticactivity, Ornithobacterium rhinotracheale, South America

Abstract

Ornithobacterium rhinotracheale is a bacterial pathogen of the poultry industry causing severe economic losses worldwide. Fifteen β-hemolytic and 23 non-hemolytic O. rhinotracheale isolates from commercial broiler and broiler breeder chicken flocks were obtained and their sensitivity to ampicillin, trimethoprim-sulfamethoxazole, gentamicin, enrofloxacin, erythromycin, florfenicol, doxycycline and fosfomycin tested by a disk diffusion method. All isolates were non-susceptible to gentamicin. Most were non-susceptible to enrofloxacin, erythromycin, trimethoprim-sulfamethoxazole, doxycycline and fosfomycin, while most were susceptible to ampicillin and florfenicol. Susceptibility to ampicillin and florfenicol was significantly higher compared to all other antibiotics tested. Overall, 34 out of 38 (89.5%) isolates were found to be multiresistant. The presence of these multiresistant O. rhinotracheale isolates is suggestive of antibiotic over and misusing in the local poultry industry. To the authors’ knowledge, this is the first work in South America reporting in vitro antibiotic susceptibility patterns of Argentine β-hemolytic and non-hemolytic O. rhinotracheale isolates.

References

Ak, S. and N. Turan, 2001. Antimicrobial susceptibility of Ornithobacterium rhinotracheale isolated from broiler chickens in Turkey. Veterinarski Arhiv, 71: 121-127.

Banani, M., S.A. Pourbakhsh and A.H. Deihimi, 2004. Antibiotic sensitivity of Ornithobacterium rhinotracheale isolates associated with respiratory diseases. Arch. Razi, 58: 111-117.

Charlton, B.R., S.E. Channing-Santiago, A.A. Bickford, C.J. Cardona and R.P. Chin et al., 1993. Preliminary characterization of a pleomorphic gram-negative rod associated with avian respiratory disease. J. Vet. Diagn. Invest., 5: 47-51.

Chin, R.P., P.C.M. van Empel and H.M. Hafez, 2013. Ornithobacterium rhinotracheale infection (ORT). In: Diseases of Poultry, Swayne D.E., J.R. Glisson, L.R. McDougald, L.K. Nolan, D.L. Suarez and V.L. Nair (Eds.), 13th Edn., Wiley-Blackwell, Ames, Iowa, USA., pp: 828-834.

CLSI., 2009. Disk diffusion: Performance standards for antimicrobial susceptibility testing. Nineteenth Informational Supplement, Clinical and Laboratory Standards Institute, Wayne, PA.

Devriese, L.A., J. Hommez, P. Vandamme, K. Kersters and F. Haesebrouck, 1995. In vitro antibiotic sensitivity of Ornithobacterium rhinotracheale strains from poultry and wild birds. Vet. Record, 137: 435-436.

Devriese, L.A., P. de Herdt and F. Haesebrouck, 2001. Antibiotic sensitivity and resistance in Ornithobacterium rhinotracheale strains from Belgian broiler chickens. Avian Pahol., 30: 197-200.

Churria, C.D.G., M.A. Machuca, G.B. Vigo and M.A. Petruccelli, 2012. Ornithobacterium rhinotracheale infection in poultry: An updated review. Int. J. Mol. Zool., 2: 23-38.

Malik, Y.S., K. Olsen, K. Kumar and S.M. Goyal, 2003. In vitro antibiotic resistance profiles of Ornithobacterium rhinotracheale strains isolated from minnesota Turkeys during 1996-2002. Avian Dis., 47: 588-593.

Mohd-Zain, Z., T.L. Jee and K. Jusoff, 2008. Phenotypic characteristics, antibiotic susceptibility and pathogenicity of Ornithobacterium rhinotracheale. WSEAS Trans. Biol. Biomed., 7: 133-142.

Murthy, T.R.G.K., N. Dorairajan, G.A. Balasubramanium, A.M. Dinakaran and K. Saravanabava, 2008. In vitro antibiotic sensitivity of Ornithobacterium rhinotracheale strains isolated from laying hens in India. Veterinarski Arhiv, 78: 49-56.

Soriano, V.E., N.A. Vera, C.R. Salado, R.P. Fernandez and P.J. Blackall, 2003. In vitro susceptibility of Ornithobacterium rhinotracheale to several antimicrobial drugs. Avian Dis., 47: 476-480.

Tsai, H.J. and C.W. Huang, 2006. Phenotypic and molecular characterization of isolates of Ornithobacterium rhinotracheale from chickens and pigeons in Taiwan. Avian Dis., 50: 502-507.

Van Veen, L., E. Hartman and T. Fabri, 2001. In vitro antibiotic sensitivity of strains of Ornithobacterium rhinotracheale isolated in The Netherlands between 1996 and 1999. Vet. Rec., 49: 611-613.

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Published

2016-07-15

Issue

Vol. 15 No. 8 (2016)

Section

Research Article

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Copyright (c) 2016 Asian Network for Scientific Information

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How to Cite

Churria , C. D. G., Loukopoulos, P., Vigo, G. B., Sansalone, P., Machuca, M. A., Nievas, V., Piscopo, M., Loyola, M. H., & Petruccelli, M. A. (2016). In vitro Antibiotic Susceptibility Patterns of Ornithobacterium rhinotracheale from Commercial Chickens in Argentina#. International Journal of Poultry Science, 15(8), 293–296. https://doi.org/10.3923/ijps.2016.293.296