Complex Hemograms of Isolator Raised Specific Pathogen Free (SPF) Chicks
DOI:
https://doi.org/10.3923/ijps.2016.211.217Keywords:
Atypia, complex hemograms, heterophil lymphocyte ratios, welfareAbstract
Heterophil/lymphocyte (H/L ratio) statistics and total white blood cell counts (TWBC) determined by light microscopic examination of blood from isolator raised specific pathogen free (SPF) chicks indicated complex hemograms. Brachial vein blood (N =12) obtained at 6 wk was spread into films, air dried, post-fixed in MeOH and Wright’s stained. H/L 1 ratios were calculated by dividing all heterophil types, typical (HT) variant (HV) and classic (HC) by the number of small (resting) lymphocytes (Ls) H/L 1 = (HT + HV + HC)/(Ls). A second ratio (H/L 2) obtained by dividing heterophils by all lymphocytes (resting, reactive and atypical, (HT + HV + HC)/ (Ls + Lm) was calculated; as was the H/L 1-H/L 2 difference (ΔH/L). TWBC’s were determined from the same films. Sorting, non-random distribution of cells and atypia affected all H/L statistics. The mean H/L 1 ratio from standard differential counts (SDC) = 0.17 and H/L 2 = 0.16. Edge based ratios (EB) were ~8 times the SDC values. Atypical cells were in all samples and more common in EB counts. The H/L ratios and ΔH/L calculated from SDC and EB values were significantly different (p<0.02). Collectively these observations support earlier conclusions regarding the lack of sensitivity of a single H/L ratio, without a TWBC, or consideration of atypia to estimate welfare. Furthermore, hemograms of SPF chickens reared in isolation may be complex.
References
Burton, R. and C.W. Guion, 1968. The differential leucocyte blood count: Its precision and individuality in the chicken. Poult. Sci., 47: 1945-1949.
Campbell, T.W., C.K. Ellis and B. Doneley, 2007. Avian and Exotic Animal Hematology and Cytology. 3rd Edn., Blackwell Publishing, Ames, Iowa, USA, Pages: 270.
Cotter, P.F., 2015. An examination of the utility of heterophil-lymphocyte ratios in assessing stress of caged hens. Poult. Sci., 94: 512-517.
Cotter, P.F., 2015. Are peripheral Mott cells an indication of stress or inefficient immunity? Poult. Sci., 94: 1433-1438.
Cotter, P.F., 2015. Atypical lymphocytes and leukocytes in the peripheral circulation of caged hens. Poult. Sci., 94: 1439-1445.
Cotter, P., D. Karcher and C. Robison, 2013. Coccinocyte: A novel lymphocyte of commercial pullets, a possible precursor to NK cells. Poult. Sci., 92: 192-193.
Cotter, P.F. and E.D. Heller, 2015. Complex hemograms in SPF chickens-An indication of cryptic infection? Poult. Sci., 94: 160-160.
Davison, T.F., J.G. Rowell and J. Rea, 1983. Effects of dietary corticosterone on peripheral blood lymphocyte and granulocyte populations in immature domestic fowl. Res. Vet. Sci., 34: 236-239.
Fleck, L., 1949. Hyperheparinemia, white emboli and leukergy. J. Am. Med. Assoc., 139: 542-542.
Gobel, T.W.F., C.L.H. Chen, J. Shrimpf, C.E. Grossin and A. Bernot et al., 1994. Characterization of avian natural killer cells and their intracellular CD3 protein complex. Eur. J. Immunol., 24: 1685-1691.
Gross, W.B., 1989. Factors affecting chicken thrombocyte morphology and the relationship with heterophil: Lymphocyte ratios. Br. Poult. Sci., 30: 919-925.
Gross, W.B. and H.S. Siegel, 1983. Evaluation of the heterophil/lymphocyte ratio as a measure of stress in chickens. Avian Dis., 27: 972-979.
Gross, W.B. and P.B. Siegel, 1986. Effects of initial and second periods of fasting on heterophil/lymphocyte ratios and body weight. Avian Dis., 30: 345-346.
Lucas, A.M. and C. Jamroz, 1961. Atlas of Avian Hematology, Issue 25. USDA, Washington DC., USA., Pages: 271.
McFarlane, J.M., S.E. Curtis, J. Simon and O.A. Izquierdo, 1989. Multiple concurrent stressors in chicks. 2. Effects on hematologic, body composition and pathologic traits. Poult. Sci., 68: 510-521.
McFarlane, J.M. and S.E. Curtis, 1989. Multiple concurrent stressors in chicks. 3. Effects on plasma corticosterone and the heterophil: Lymphocyte ratio. Poult. Sci., 68: 522-527.
Otremski, I., R.J. Newman, P.J. Kahn, J. Stadler, N. Kariv, Y. Skornik and G. Goldman, 1993. Leukergy-a new diagnostic test for bone infection. J. Bone Joint Surg., 75: 734-736.
Reagan, W.J., A.R. Irizarry-Rovira and D.B. DeNicola, 2008. Veterinary Hematology, Atlas of Common Domestic and Non-Domestic Species. 2nd Edn., Wiley-Blackwell Publication, Ames, IA., USA.
Rebar, A.H. and R.E. Raskin, 2006. Cytology of effusions. Proceedings of the 31st Congress of the World Small Animal Veterinary Association, October 11, 2006, Prague, Czech Republic, pp: 659-666.
Douglas, J.W. and K.J. Wardrop, 2010. Schalm's Veterinary Hematology. 6th Edn., Wiley-Blackwell, London, UK., ISBN: 9780813817989, Pages: 1232.
Wolford, J.H. and R.K. Ringer, 1962. Adrenal weight, adrenal ascorbic acid, adrenal cholesterol and differential leucocyte counts as physiological indicators of stressor agents in laying hens. Poult. Sci., 41: 1521-1529.
Yan, F.F., P.Y. Hester and H.W. Cheng, 2014. The effect of perch access during pullet rearing and egg laying on physiological measures of stress in White Leghorns at 71 weeks of age. Poult. Sci., 93: 1318-1326.
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