Influence of Dietary Enzymes Prepared at Ensiling (ZADO®) from Hatch to 42 Days of Age on Productivity, Slaughter Traits and Blood Constituents in Broiler Chickens
DOI:
https://doi.org/10.3923/ijps.2013.529.537Keywords:
Blood biochemical characteristics, broiler performance, slaughter traits, ZADO enzyme complexAbstract
Four hundred Cobb-500 broiler chicks at one-day old were used to study the effects of exogenous xylanases, cellulases, protease and alpha-amylase enzyme preparations at ensiling (ZADO®) on the productive performance, slaughter traits and blood metabolites. Chicks were divided randomly into 4 treatments (broiler basal diets supplemented with 0, 2, 4 and 6‰ with ZADO®) and housed on deep litter at an open house system under commercial conditions. Each treatment replicated 4 times (30 chicks per replicate). Basal diet contained 23.1% CP and 3,103 kcal AME/kg for the starter diet (0-21 d) and 20.0% CP and 3,207 kcal AME/kg for the finisher diet (21-42 d). Results indicated that broiler productivity improved in response to dietary 4 or 6‰ ZADO®. Feed conversion ratio was 1.948, 1.903, 1.758 and 1.678 for birds fed diet supplemented with 0, 2, 4 and 6‰ ZADO® (p = 0.0007), respectively. In addition, birds fed diet supplemented with 6‰ ZADO® recorded higher dressing and immune organs relative weights at 42 days of age than other treatments (p<0.05). Moreover, enzyme supplementation increased significantly plasma total protein (p = 0.0040) and globulin (p = 0.0131) at 42 days of age. Also, dietary 6‰ ZADO® decreased plasma cholesterol (p = 0.0466) and increased HDL-:Total-cholesterol ratio (p = 0.0040). It could be concluded that ZADO® supplementation to broiler diets improved broiler productivity and might improve immunity. Moreover, dietary 6‰ ZADO® has a favor effects on lipid metabolism. It could be recommended from this study to supplement 4‰ or more of ZADO® to broiler diets up to 42 days of age.
References
Allain, C.C., L.S. Poon, C.S.G. Chan, W. Richmond and P.C. Fu, 1974. Enzymatic determination of total serum cholesterol. Clin. Chem., 20: 470-475.
Angel, C.R., W. Saylor, S.L. Vieira and N. Ward, 2011. Effects of a monocomponent protease on performance and protein utilization in 7- to 22-day-old broiler chickens. Poult. Sci., 90: 2281-2286.
AOAC., 2000. Official Method of Analysis of the Association of Official Analytical Chemists. 17th Edn., AOAC International, Gaithersburg, MD., USA.
Barekatain, M.R., C. Antipatis, M. Choct and P.A. Iji, 2013. Interaction between protease and xylanase in broiler chicken diets containing sorghum distillers' dried grains with solubles. Anim. Feed Sci. Technol., 182: 71-81.
Bedford, M.R., 2002. The Role of Carbohydrases in Feedstuff Digestion. In: Poultry Feedstuffs: Supply, Composition and Nutritive Value, McNab, J.M. and K.N. Boorman (Eds.). CAB International, Edinburgh, UK., pp: 319-336.
Cafe, M.B., C.A. Borges, C.A. Fritts and P.W. Waldroup, 2002. Avizyme improves performance of broilers fed corn-soybean meal-based diets. J. Applied Poult. Res., 11: 29-33.
Cowieson, A.J. and V. Ravindran, 2008. Effect of exogenous enzymes in maize-based diets varying in nutrient density for young broilers: Growth performance and digestibility of energy, minerals and amino acids. Br. Poult. Sci., 49: 37-44.
Cowieson, A.J., D.N. Singh and O. Adeola, 2006. Prediction of ingredient quality and the effect of a combination of xylanase, amylase, protease and phytase in the diets of broiler chicks. 1. Growth performance and digestible nutrient intake. Br. Poult. Sci., 47: 477-489.
Cowieson, A.J., A. Ptak, P. Mackowiak, M. Sassek and E. Pruszynska-Oszmalek et al., 2013. The effect of microbial phytase and myo-inositol on performance and blood biochemistry of broiler chickens fed wheat/corn-based diets. Poult. Sci., 92: 2124-2134.
Del Bianchi, M., C.A.F. Oliveira, R. Albuquerque, J.L. Guerra and B. Correa, 2005. Effects of prolonged oral administration of aflatoxin B1 and fumonisin B1 in broiler chickens. Poult. Sci., 84: 1835-1840.
Donaldson, W.E., 1985. Lipogenesis and body fat in chicks: Effects of calorie-protein ratio and dietary fat. Poult. Sci., 64: 1199-1204.
Donaldson, W.E., G.F. Combs and G.L. Romoser, 1958. Studies on energy levels in poultry rations. 1. Effect of calorie-protein ratio of the ration on growth, nutrient utilization and body composition of chicks. Poult. Sci., 35: 1100-1105.
Doumas, B.T., W. Watson and H.G. Biggs, 1971. Albumin and globulin standards measurements by direct colourimetric method with Bromo Cresol Green (BCG) in serum or plasma. Clin. Chem. Acta, 31: 87-96.
El-Sayed, D.A.A., A.M. Abdou, S.M.M. Shalash, H.M. Safaa and S.A. Riad, 2011. Productivity and immune response of broiler chickens vaccinated with different Avian Influenza vaccines at one or seven days of age. Aust. J. Basic Applied Sci., 5: 325-334.
Freitas, D.M., S.L. Vieira, C.R. Angel, A. Favero and A. Maiorka, 2011. Performance and nutrient utilization of broilers fed diets supplemented with a novel mono-component protease. J. Applied Poult. Res., 20: 322-334.
Frigard, T., D. Pettersson and P. Aman, 1994. Fiber-degrading enzyme increases body weight and total serum cholesterol in broiler chickens fed a rye-based diet. J. Nutr., 124: 2422-2430.
Gado, H., 1997. Effect of enzymatic treatments for poor quality roughages on fiber digestibility and nitrogen metabolism in Baladi goats. Egypt. J. Nutr. Feeds, 1: 50-56.
Gado, H.M. and A.Z.M. Salem, 2008. Influence of exogenous enzymes from anaerobic source on growth performance, digestibility, ruminal fermentation and blood metabolites in lambs fed of orange pulp silage in total mixed ration. Proceedings of the 59th Annual Meeting of the European Association for Animal Production, August 24-27, 2008, Vilnius, Lithuania, pp: 228-230.
Gado, H.M., H.M. Metwally, H. Soliman, A.Z.L. Basiony and E.R. El-Galil, 2007. Enzymatic treatments of bagasse by different sources of cellulase enzymes. Proceedings of the 11th Conference on Animal Nutrition, Volume 2, November 13-18, 2007, Egypt, pp: 13-18.
Gado, H.M., A.Z.M. Salem, P.H. Robinson and M. Hassan, 2009. Influence of exogenous enzymes on nutrient digestibility, extent of ruminal fermentation as well as milk production and composition in dairy cows. Anim. Feed Sci. Technol., 154: 36-46.
Gado, H.M., A.Z.M. Salem, N.E. Odongo and B.E. Borhami, 2011. Influence of exogenous enzymes ensiled with orange pulp on digestion and growth performance in lambs. Anim. Feed Sci. Technol., 165: 131-136.
Gao, F., Y. Jiang, G.H. Zhou and Z.K. Han, 2007. The effects of xylanase supplementation on growth, digestion, circulating hormone and metabolite levels, immunity and gut microflora in cockerels fed on wheat-based diets. Br. Poult. Sci., 48: 480-488.
Gornall, A.G., C.J. Bardawill and M.M. Divid, 1949. Biuret colourimetric method in plasma. J. Biol. Chem., 177: 751-766.
Gracia, M.I., M.J. Aranibar, R. Lazaro, P. Medel and G.G. Mateos, 2003. Alpha-amylase supplementation of broiler diets based on corn. Poult. Sci., 82: 436-442.
Iji, P.A., A. Saki and D.R. Tivey, 2001. Body and intestinal growth of broiler chicks on a commercial starter diet. 1. Intestinal weight and mucosal development. Br. Poult. Sci., 42: 505-513.
Kalmendal, R. and R. Tauson, 2012. Effects of a xylanase and protease, individually or in combination and an ionophore coccidiostat on performance, nutrient utilization and intestinal morphology in broiler chickens fed a wheat-soybean meal-based diet. Poult. Sci., 91: 1387-1393.
Khalaji, S., M. Zaghari, K.H. Hatami, S. Hedari-Dastjerdi, L. Lotfi and H. Nazarian, 2011. Black cumin seeds, Artemisia leaves (Artemisia sieberi) and Camellia L. plant extract as phytogenic products in broiler diets and their effects on performance, blood constituents, immunity and cecal microbial population. Poult. Sci., 90: 2500-2510.
Kocher, A., M. Choct, M.D. Porter and J. Broz, 2002. Effects of feed enzymes on nutritive value of soybean meal fed to broilers. Br. Poult. Sci., 43: 54-63.
Kocher, A., M. Choct, G. Ross, J. Broz and T.K. Chung, 2003. Effects of enzyme combinations on apparent metabolizable energy of corn-soybean meal-based diets in broilers. J. Applied Poult. Res., 12: 275-283.
Lazaro, R., M. Garcia, M.J. Aranibar and G.G. Mateos, 2003. Effect of enzyme addition to wheat-, barley- and rye-based diets on nutrient digestibility and performance of laying hens. Br. Poult. Sci., 44: 256-265.
Lopes-Virella, M.F., P. Stone, S. Ellis and J.A. Colwell, 1977. Cholesterol determination in high-density lipoproteins separated by three different methods. Clin. Chem., 23: 882-884.
Mabray, C.J. and P.W. Waldroup, 1981. The influence of dietary energy and amino acid levels on abdominal fat pad development of the broiler chicken. Poult. Sci., 60: 151-159.
Marsman, G.J., H. Gruppen, A.F. van der Poel, R.P. Kwakkel, M.W. Verstegen and A.G. Voragen, 1997. The effect of thermal processing and enzyme treatments of soybean meal on growth performance, ileal nutrient digestibilities and chyme characteristics in broiler chicks. Poult. Sci., 76: 864-872.
Mathlouthi, N., S. Mallet, L. Saulnier, B. Quemener and M. Larbier, 2002. Effect of xylanase and β-glucanase addition on performance, nutrient digestibility and physico-chemical conditions in the small intestine contents and caecal microflora of broiler chickens fed a wheat and barley-based diet. Anim. Res., 51: 395-406.
Meluzzi, A., G. Primiceri, R. Giordani and G. Fabris, 1992. Determination of blood constituents reference values in broilers. Poult. Sci., 71: 337-345.
Meng, X., B.A. Slominski, L.D. Campbell, W. Guenter and O. Jones, 2006. The use of enzyme technology for improved energy utilization from full-fat oilseeds. Part I: Canola seed. Poult. Sci., 85: 1025-1030.
NRC., 1994. Nutrient Requirements of Poultry. 9th Edn., National Academy Press, Washington, DC., USA., ISBN-13: 9780309048927, Pages: 176.
Olukosi, O.A., A.J. Cowieson and O. Adeola, 2007. Age-related influence of a cocktail of xylanase, amylase and protease or phytase individually or in combination in broilers. Poult. Sci., 86: 77-86.
Onilude, A.A. and B.A. Oso, 1999. Effect of fungal enzyme mixture supplementation of various fibre-containing diets fed to broiler chicks 1: Performance and carcass characteristics. World J. Microbiol. Biotechnol., 15: 309-314.
Onilude, A.A. and B.A. Oso, 1999. Effect of fungal enzyme mixture supplementation of various fibre-containing diets fed to broiler chicks 2: On blood, liver and kidney total lipids, triacylglycerols and cholesterol. World J. Microbiol. Biotechnol., 15: 315-320.
Peek, H.W., J.D. van der Klis, B. Vermulen and W.J.M. Landman, 2009. Dietary protease can alleviate negative effects of a coccidiosis infection on production performance in broiler chickens. Anim. Feed Sci. Technol., 150: 151-159.
Reitman, S. and S. Frankel, 1957. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol., 28: 56-63.
Safaa, H.M., S.A. Riad, F.R. Mohamed, S.S. Siam and H.A. El-Minshawy, 2010. Probiotic, prebiotic and yeast supplementation in broiler diets from 1 to 42 days of age: 2. Immune response and slaughter traits. Poult. Sci., 89: 546-547.
Saleh, F., M. Tahir, A. Ohtsuka and K. Hayashi, 2005. A mixture of pure cellulase, hemicellulase and pectinase improves broiler performance. Br. Poult. Sci., 46: 602-606.
Sarica, S., A. Ciftci, E. Demir, K. Kilinc and Y. Yildirim, 2005. Use of an antibiotic growth promoter and two herbal natural feed additives with and without exogenous enzymes in wheat based broiler diets. S. Afr. J. Anim. Sci., 35: 61-72.
SAS., 2004. SAS/STATs User's Guide. Version 9.1, SAS Institute Inc., Cary, NC., USA.
Snedecor, G.W. and W.G. Cochran, 1967. Statistical Methods. 6th Edn., Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi, India, Pages: 593.
Steel, R.G.D. and J.H. Torrie, 1980. Principles and Procedures of Statistics: A Biometrical Approach. 2nd Edn., McGraw-Hill, New York, United State, ISBN: 9780070609266, Pages: 633.
Steinberg, D., 1981. Metabolism of Lipoproteins at the Cellular Level in Relation to Atherogenesis. In: Lipoproteins, Atherosclerosis and Coronary Heart Disease, Miller, N.E. and B. Lewis (Eds.). Elsevier, The Netherlands, pp: 31-48.
Viveros, A., A. Brenes, I. Arija and C. Centeno, 2002. Effects of microbial phytase supplementation on mineral utilization and serum enzyme activities in broiler chicks fed different levels of phosphorus. Poult. Sci., 81: 1172-1183.
Walk, C.L., A.J. Cowieson, J.C. Remus, C.L. Novak and A.P. McElroy, 2011. Effects of dietary enzymes on performance and intestinal goblet cell number of broilers exposed to a live coccidia oocyst vaccine. Poult. Sci., 90: 91-98.
Zanella, I., N.K. Sakomura, F.G. Silversides, A. Fiqueirdo and M. Pack, 1999. Effect of enzyme supplementation of broiler diets based on corn and soybeans. Poult. Sci., 78: 561-568.
Zhang, G.F., Z.B. Yang, Y. Wang, W.R. Yang, S.Z. Jiang and G.S. Gai, 2009. Effects of ginger root (Zingiber officinale) processed to different particle sizes on growth performance, antioxidant status and serum metabolites of broiler chickens. Poult. Sci., 88: 2159-2166.
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