Efficacy of Eimeria tenella (Oocyst and Sporozoite) Proteins as a Vaccine in Broilers Against Coccidiosis
DOI:
https://doi.org/10.3923/ijps.2013.157.163Keywords:
Eimeria tenella, oocyst, sporozoite, vaccineAbstract
The protective efficacy against homologous challenge in chickens evaluated by using two proteins of a (Sporozoite) and (Oocyst) from Eimeria tenella. Immunization was applied on 3rd and 16th day of age subcutaneously with this two types of protein in separate groups at dose (25μg per chicken). Vaccinated birds were challenged at 30th day of age, demonstrated that sporozoite protein could provide chickens with protection rate around 99.2-99.5%, while oocyst protein gives 67-69% protection, number of oocysts and cecal lesion from chickens in the immunized groups with sporozoite protein decrease significantly and this protein was more effective from another groups. The body weight gain not affected (higher) in sporozoite immunized groups when compared with oocyst protein immunized groups and control positive groups, also we estimated ACI which was demonstrated that sporozoite protein was very effective while the oocyst protein was slightly effective.
References
Al-Attar, M.A. and R.M. Al-Qshtiny, 1996. Attention of Eimeria tenella parasite by us gamma rays for the immunization purposes in chickens. Proceedings of the 3rd Arab Conference for the Uses of Atomic Energy, (UAE'96), Damascus, Syria.
Allen, P.C. and R.H. Fetterer, 2002. Recent advances in biology and immunobiology of Eimeria species and in diagnosis and control of infection with these coccidian parasites of poultry. Clin. Microbiol. Rev., 15: 58-65.
Badawy, G.A. and M.G. Aggour, 2006. Immune responses in chickens against Eimeria tenella antigen. Assiut. Vet. Med. J., 52: 178-186.
Brothers, V.M., I. Kuhn, L.S. Paul, J.D. Gabe and W.H. Andrews et al., 1988. Characterization of a surface antigen of Eimeria tenella sporozoites and synthesis from a cloned cDNA in Escherichia coli. Mol. Biochem. Parasitol., 28: 235-247.
Chapman, H.D., B. Roberts, M.W. Shirley and R.B. Williams, 2005. Guidelines for evaluating the efficacy and safety of live anticoccidial vaccines and obtaining approval for their use in chickens and turkeys. Rev. Avian Pathol., 34: 279-290.
Conway, D.P. and M.E. Mckenzie, 2007. Poultry Coccidiosis Diagnostic and Testing Procedure. 3rd Edn., Wiley-Blackwell, London, UK., ISBN: 9780813822020, pp: 42-44.
Danforth, H.D. and McAndew, 1987. Hybridoma antibody characterization of stage-specific and stage-cross-reactive antigens of Eimeria tenella. J. Parasitol., 73: 985-992.
Dulski, P. and M. Turner, 1987. The purification of sporocysts and sporozoites from Eimeria tenella Oocysts using percoll density gradients. Avian. Dis., 32: 235-239.
Geriletu, L. Xu, Xurihua and X. Li, 2011. Vaccination of chickens with DNA vaccine expressing Eimeria tenella MZ5-7 against coccidiosis. Vet. Parasitol., 177: 6-12.
Johnson, J. and W.M. Reid, 1970. Anticoccidial drugs: Lesion scoring techniques in battery and floor-pen experiments with chickens. Exp. Parasitol., 28: 30-36.
Karkhanis, Y.D., K.A. Nollstadt, B.S. Bhogal, O.W.E.N. Ravino and R. Pellegrino et al., 1991. Purification and characterization of a protective antigen from Eimeria tenella. Infect. Immun., 59: 983-989.
Kodama, Y., H. Yokoyoman and S.V. Nguyen, 2006. Compositions against chicken coccidiosis. US. Patent Application Puplication WO 2004002527 A1.
Kussibati, R., R. Al-Monaged, H. Tarsha and A.M. Subuh, 2003. Nutrition for Poultry and Animals. Al-Baath University, Homs, Syria, pp: 181-182.
Li, J., J. Zheng, P. Gong and X. Zhang, 2012. Efficacy of Eimeria tenella rhomboid-like protein as a subunit vaccine in protective immunity against homologous challenge. Parasitol. Res., 110: 1139-1145.
Long, P.L., B.J. Millard, L.P. Joyner and C.C. Norton, 1976. A guide to laboratory techniques used in the study and diagnosis of avian coccidiosis. Folia Vet. Lat., 6: 201-217.
Mansoori, B. and M. Modirsanei, 2012. Effects of dietary tannic acid and vaccination on the course of coccidiosis in experimentally challenged broiler chicken. Vet. Parasitol., 1871: 119-122.
Miller, T.J., L. Nebr, R.A. Clare, M. Pa, P. Lufburrow and S. Calif, 1998. Continuos cell line and vaccine against avian coccidia. United State Patent 5846527 A.
Murray, P.K. and S. Galuska, 1986. Coccidiosis vaccine. European Patent Application No. 0167442 A2.
Riggs, M.W. and L.E. Perryman, 1987. Infectivity and neutralization of Cryptosporidium parvum sporozoites. Infect. Immunol., 5519: 2081-2087.
Schamatz, D.M., M.S.J. Crane and P.K. Murrey, 1984. Purification of Eimeria sporozoites by DE-52 anion exchange chromatography. J. Protozool., 31: 181-183.
Sharman, P.A., N.C. Smith, M.G. Wallach and M. Katrib, 2010. Chasing the golden egg: Vaccination against poultry coccidiosis. Parasite Immunol., 32: 590-598.
Subramanian, B.M., R. Sriraman, N.H. Rao, J. Raghul, D. Thiagarajan and V.A. Srinivasan, 2008. Cloning, expression and evaluation of the efficacy of a recombinant Eimeria tenella sporozoite antigen in birds. Vaccine, 26: 3489-3496.
Vermeulen, A.N., 1998. Progress in recombinant vaccine development against coccidiosis A review and prospects into the next millennium. Int. J. Parasitol., 28: 1121-1130.
Vermeulen, A.N., D.C. Schaap and T.P.M. Schetters, 2001. Control of coccidiosis in chickens by vaccination. Vet. Parasitol., 100: 13-20.
Wallach, M., N.C. Smith, C.M. Miller, J. Eckert and M.E. Rose, 1994. Eimeria maxima: ELISA and western blot analyses of protective sera. Parasite Immunol., 16: 377-378.
Williams, R.B., 1999. A compartment aliased model for the estimation the cost of coccidiosis to the world's chicken production industry. Int. J. Parasitol., 29: 1209-1229.
Williams, R.B., 2002. Anticoccidial vaccines for broiler chickens: Pathways to success. Avian Pathol., 31: 317-353.
Williams, R.B. and J. Catchpole, 2000. A new protocol for a challenge test to assess the efficacy of live anticoccidial vaccines for chickens. Vaccine, 18: 1178-1185.
Yang, J.F., R.Q. Wang, R.Q. Lv, D.H. Zhou, G. Duan and F.C. Zou, 2012. Anticoccidial activity of Eupatorium adenophorum extracts against chicken coccidian Oocysts. J. Anim. Vet. Adv., 11: 1255-1257.
Downloads
Published
Issue
Section
License
Copyright (c) 2013 Asian Network for Scientific Information
This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
