Genomic Organization and Promoter Characterization of RANTES, MIP-1ß like and MCP-Like CC Chemokines of Domestic Duck (Anas platyrhynchos)
DOI:
https://doi.org/10.3923/ijps.2009.1123.1127Keywords:
CC chemokine, duck, MCP, MIP-1β, RANTESAbstract
The duck immunology is gaining more interest in recent years since ducks have been identified as “Trojan horse ” of highly pathogenic avian influenza. In the present study, we characterize the gene and promoter regions of three CC chemokines from domestic duck. Analysis of the gene revealed significant similarity with chicken and mammalian chemokine genes with three coding exon and two intron pattern. Though the exons are highly conserved with respect to corresponding chicken chemokine genes, introns revealed low identity. Promoter regions of duck RANTES and MCP-like chemokines were amplified by Genome walking method. Analysis of the 500 bp upstream nucleotide sequences of ATG (translational start site) of RANTES and MCP-like chemokine revealed a number of putative binding sites for transcription factors. Comparative analysis of RANTES promoter with human and chicken sequences revealed conserved TATA box, NFkB binding site and transcription start site. The predicted transcription start site and TATA box are also conserved in duck and chicken MCP-like chemokine sequences. The present study shows that the over all organization of gene and putative transcription factor binding sites in the promoter regions of these three chemokines were conserved with respect to chicken and human sequences indicating these molecules of innate immune system is conserved in the process of evolution.
References
Asif, M., K.A. Jenkins, L.S. Hilton, W.G. Kimpton, A.G. Bean and J.W. Lowenthal, 2004. Cytokines as adjuvants for avian vaccines. Immunol. Cell Biol., 82: 638-643.
Baggiolini, M., 1998. Chemokines and leukocyte traffic. Nature, 392: 565-568.
Boehlk, S., S. Fessele, A. Mojaat, N.G. Miyamoto and T. Werner et al., 2000. ATF and Jun transcription factors, acting through an Ets/CRE promoter module, mediate lipopolysaccharide inducibility of the chemokine RANTES in monocytic Mono Mac 6 cells. Eur. J. Immunol., 30: 1102-1112.
Casola, A., R.P. Garofalo, H. Haeberle, T.F. Elliott, R. Lin, M. Jamaluddin and A.R. Brasier, 2001. Multiple cis regulatory elements control RANTES promoter activity in alveolar epithelial cells infected with respiratory syncytial virus. J. Virol., 75: 6428-6439.
Genin, P., M. Algarte, P. Roof, R. Lin and J. Hiscott, 2000. Regulation of RANTES chemokine gene expression requires cooperativity between NF-kappa B and IFN-regulatory factor transcription factors. J. Immunol., 164: 5352-5361.
Goulding, C., R. McManus, A. Murphy, G. MacDonald and S. Barrett et al., 2005. The CCR5-delta 32 mutation: Impact on disease outcome in individuals with hepatitis C infection from a single source. Gut, 54: 1157-1161.
Hilton, L.S., A.G. Bean, W.G. Kimpton and J.W. Lowenthal, 2002. Interleukin-2 directly induces activation and proliferation of chicken T cells in vivo. J. Interferon Cytokine Res., 22: 755-763.
Hughes, A.L., 1997. Rapid evolution of immunoglobulin super family C2 domains expressed in immune system cells. Mol. Biol. Evol., 14: 1-5.
Hughes, S., T.Y. Poh, N. Bumstead and P. Kaiser, 2007. Re-evaluation of the chicken MIP family of chemokines and their receptors suggests that CCL5 is the prototypic MIP family chemokine and that different species have developed different repertoires of both the CC chemokines and their receptors. Dev. Comp. Immunol., 31: 72-86.
Hulse-Post, D.J., K.M. Sturm-Ramirez, J. Humberd, P. Seiler and E.A. Govorkova et al., 2005. Role of domestic ducks in the propagation and biological evolution of highly pathogenic H5N1 influenza viruses in Asia. Proc. Natl. Acad. Sci. USA., 102: 10682-10687.
Kishida, N., Y. Sakoda, N. Isoda, K. Matsuda and M. Eto et al., 2005. Pathogenicity of H5 influenza viruses for ducks. Arch. Virol., 150: 1383-1392.
Kujime, K., S. Hashimoto, Y. Gon, K. Shimizu and T. Horie, 2000. p 38 mitogen-activated protein kinase and c-jun-NH2-terminal kinase regulate RANTES production by influenza virus-infected human bronchial epithelial cells. J. Immunol., 164: 3222-3228.
Liu, H., D. Chao, E.E. Nakayama, H. Taguchi and M. Goto et al., 1999. Polymorphism in RANTES chemokine promoter affects HIV-1 disease progression. Proc. Natl. Acad. Sci. USA., 968: 4581-4585.
Melchjorsen, J., L.N. Sorensen and S.R. Paludan, 2003. Expression and function of chemokines during viral infections: from molecular mechanisms to in vivo function. J. Leukocyte Biol., 74: 331-343.
Miller, S.A., D.D. Dykes and H.F. Polesky, 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res., 16: 1215-1215.
Murphy, P.M., 1993. Molecular mimicry and the generation of host defense protein diversity. Cell, 72: 823-826.
Nelson, P.J., B.D. Ortiz, J.M. Pattison and A.M. Krensky, 1996. Identification of a novel regulatory region critical for expression of the RANTES chemokine in activated T lymphocytes. J. Immunol., 157: 1139-1148.
Olsen, S.J., K. Ungchusak, M. Birmingham, J. Bresee, S.F. Dowell and S. Chunsuttiwat, 2006. Surveillance for avian influenza in human beings in Thailand. Lancet Infect. Dis., 6: 757-758.
Park, B.L., Y.J. Kim, H.S. Cheong, L.H.Kim, Y.H. Choi, H.S. Lee and H.D. Shin, 2006. Association of common promoter polymorphisms of MCP1 with hepatitis B virus clearance. Exp. Mol. Med., 38: 694-702.
Schultz, U., E. Grgacic and M. Nassal, 2004. Duck hepatitis B virus: An invaluable model system for HBV infection. Adv. Virus Res., 63: 1-70.
Sreekumar, E., A. Premraj, D.S. Arathy and T.J. Rasool, 2005. Identification, sequence characterization and analysis of expression profiles of three novel CC chemokines from domestic duck (Anas platyrhynchos). Immunogenetics, 57: 364-373.
Thompson, J.D., D.G. Higgins and T.J. Gibson, 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res., 22: 4673-4680.
Toka, F.N., C.D. Pack and B.T. Rouse, 2004. Molecular adjuvants for mucosal immunity. Immunol. Rev., 199: 100-112.
Toka, F.N., M. Gierynska and B.T. Rouse, 2003. Codelivery of CCR7 ligands as molecular adjuvants enhances the protective immune response against herpes simplex virus type 1. J. Virol., 77: 12742-12752.
Wang, J., D.L. Adelson, A. Yilmaz, S.H. Sze, Y. Jin and J.J. Zhu, 2005. Genomic organization, annotation and ligand-receptor inferences of chicken chemokines and chemokine receptor genes based on comparative genomics. BMC Genomics, 6: 45-45.
Webster, R.G., W.J. Bean, O.T. Gorman, T.M. Chambers and Y. Kawaoka, 1992. Evolution and ecology of influenza A viruses. Microbiol. Rev., 56: 152-179.
Xia, J., C. Radford, X. Guo and K.E. Magor, 2007. Immune gene discovery by expressed sequence tag analysis of spleen in the duck (Anas platyrhynchos). Dev. Comp. Immunol., 31: 272-285.
Zlotnik, A., O. Yoshie and H. Nomiyama, 2006. The chemokine and chemokine receptor superfamilies and their molecular evolution. Genome Biol., 7: 243-243.
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