Protective Effect of In ovo Vaccination with IBV-Spike-Recombinant DNA and Chicken Interferon as an Adjuvant
DOI:
https://doi.org/10.3923/ijps.2009.1034.1041Keywords:
Chicken interferon alpha, infectious bronchitis virus, In ovo, recombinant DNA vaccineAbstract
Infectious Bronchitis Virus (IBV) can cause high mortality, poor weight gain and low feed efficiency of infected chickens, which leading to considerable economic losses to the poultry industry. In this study, a plasmid DNA pTracer-CVM2-IBVS (abbr: pCMV-S) that expresses the immunogenic S glycoprotein genes of IBV serotype Massachusetts 41 was inoculated in ovo into 18-days-old embryonating Specific-Pathogen-Free (SPF) chicken eggs as a vaccine. Recombinant chicken interferon type I alpha (rChIFN-α) was added as an adjuvant to enhance the immunogenicity of the vaccine. After hatch, birds were challenged at 4 weeks of age with a homologous pathogenic Massachusetts 41 field type IBV. Birds receiving a single dose of pCMV-S vaccine in ovo showed mild clinical signs and were protected at the level of 66%, whereas groups receiving a combination of the pCMV-S with 2000 IU or 500 IU of rChIFN-α showed protection at the level of 83 and 89% respectively. The pCMV-S + 500 IU rChIFN-α vaccinated group, when boosted with a live attenuated commercial IBV vaccine at 2 weeks of age indicated a significant protection (> 92 %) against IBV challenge. At 5 days post challenge, no clinical signs were seen from the birds vaccinated with 5 ug of pCMV-S +500 IU rChIFN and the birds received booster with commercial IBV vaccine. RT-PCR based diagnostic test indicated detecting IBV infection was reduced in birds received pCMV-S with 2000 IU or 500 IU of rCHIFN-α. This study indicates that the application of rChIFN-α as an adjuvant with pTracer-CMV-IBVS vaccine expressing S glycoprotein gene was somewhat effective in controlling IBV infection. Additionally an increase in protection against the homologous IBV challenge was observed in birds boosted with the live attenuated IBV commercial vaccine.
References
Asif, M., A.K. Jenkins, S.L. Hilton, G.W. Kimpton, G.A. Bean and W.J. Lowenthal, 2004. Cytokines as adjuvant for avian vaccines. Immunol. Cell Biol., 82: 638-643.
Boursnell, M.E.G., T.D.K. Brown, I.J. Foulds, P.F. Green, F.M. Tomley and M.M. Binns, 1987. Completion of the sequence of the genome of the Coronavirus avian infectious bronchitis virus. J. Gen. Virol., 68: 55-77.
Cavanagh, D., 1983. Structural characterization of the spike protein. J. Gen. Virol., 64: 2577-2583.
Cavanagh, D. and S. Naqi, 1997. Infectious Bronchitis. In: Diseases of Poultry, Calnek, B.W., H.J. Barnes, L.R. McDougald and Y.M. Saif (Eds.). 10th Edn., Iowa State University Press, London, pp: 511-526.
Dhinakar, R.G. and R.C. Jones, 1997. Infectious bronchitis virus: Immunopathogenesis of infection in the chicken. Avian Pathol., 26: 677-706.
Fabis, J.J. and M.I. Khan, 2004. In vivo expression of IBV-S gene in chicks inoculated with recombinant DNA vaccine in ovo. Proceedings of the 5th Symposium on Avian Corona and Pneumovirus Infections, June 20-23, Rauischholzhausen, Germany, pp: 232-236.
Fynan, E.F., H.L. Robinson and R.G. Webster, 1993. Use of DNA encoding influenza hemagglutinin as an avian influenza vaccine. DNA Cell Biol., 9: 785-789.
Gelb, J.J. and M.W. Jackwood, 1998. Infectious Bronchitis. In: A Laboratory Manual for the Isolation and Identification of Avian Pathogens, Swayne, D.E., J.R. Glisson, M.W. Jackwood, J.E. Pearson and W.M. Reed (Eds.). 4th Edn., American Association of Avian Pathologists, Kennett Square, PA., pp: 169-174.
Godeke, G., C.A.M. de Haan, J.W.A. Rossen, H. Vennema and P.J.M. Rottier, 2000. Assembly of spikes into corona virus particles is mediated by the carboxy-terminal domain of the spike protein. J. Virol., 74: 1566-1571.
Ignjatovic, J., G. Gould and S. Sapats, 2003. Isolation of a variant infectious bronchitis virus in Australia that further illustrates diversity among emerging strains. Arch. Virol., 151: 1567-1585.
Jarosinski, W.K., J. Wei, M.J. Sekellick and P.I. Marccus, 2001. Cellular responses in chickens treated with IFN- alpha orally or inoculated with recombinant Marek's disease virus expressing IFN-alpha. J. Interferon Cytokine Res., 21: 287-296.
Johnson, M.A., C. Pooley, J. Ignjatovic and S.G. Tyack, 2003. A recombinant fowl adenovirus expressing the S1 gene of infectious bronchitis virus protects against challenge with infectious bronchitis virus. Vaccine, 21: 2730-2736.
Kant, A., G. Koch, D.J. van Roozelaar, J.G. Kusters, F.A.J. Poelwijk and B.A.M. van der Zeijst, 1992. Location of antigenic sites defined by neutralizing monoclonal antibodies on the S1 avian infectious bronchitis virus glycopolypeptide. J. Gen. Virol., 73: 591-596.
Kapczynski, R.D., D.A. Hilt, D. Shapiro, H.S. Sellers and M.W. Jack wood, 2003. Protection of chicken from infectious bronchitis by in ovo and intramuscular vaccination with a DNA vaccine expressing the S1 glycoprotein. Avian Dis., 47: 272-285.
Khan, M.I. and J.J. Fabis, 2002. In ovo Vaccination for IBV, Using DNA Vaccine. A preliminary study. Proceedings of the 139th Annual American Veterinary Medical Association Convention and Meeting, July 13-17, Nashville, New York, pp: 73-73.
Le Bon, A., G. Schiavoni, G. D'Agostino, I. Gresser, F. Belardelli and D.F. Tough, 2001. Type I interferon potently enhance humoral immunity and can promote isotype switching by stimulating dendritic cells in vivo. Immunity, 14: 461-470.
Lowenthal, J.W., T.E. O'Neil, M. Broadway, A.D. Strom, M.R. Digby, M. Andrew and J.J. York, 1998. Co administration of IFN-gamma enhances antibody response in chickens. J. Interferon Cytokine Res., 18: 617-622.
Marcus, P.I., L. van der Heide and M.J. Sekellick, 1999. Interferon action on avian viruses. I. Oral administration of chicken interferon-alpha ameliorates Newcastle disease. J. Interferon Cytokine Res., 19: 881-885.
Moore, K.M., J.D. Bennett, B.S. Seal and M.W. Jackwood, 1998. Sequence comparison of avian infectious bronchitis virus S1 glycoproteins of the Florida serotype and five variant isolates from Georgia and California. Virus Genes, 17: 63-83.
Oshop, G.L., S. Elankumaran and R.A. Hectert, 2002. Mimi review: DNA vaccination in the avian. Vet. Imunol. Immunopathol., 89: 1-12.
Oshop, G.L., S. Elankumaran, N.V. Vakharia and R.A. Hectert, 2003. In ovo delivary of DNA to the avian embryo. Vaccine, 21: 1275-1281.
Rautenschlein, S., J.M. Sharme, B.J. Winslow, J. McMillen, D. Junker and M. Cochran, 1999. Embryo vaccination of turkeys against Newcastle disease infection with recombinant fowlpox virus constructs containing interferons as adjuvants. Vacccine, 18: 426-433.
Schijns, V.E.C., K.C. Weiningb, P. Nuijtenc, E.O. Rijked and P. Staehelib, 2000. Immunoadjuvant activities of E. coli-and plasmid-expressed recombinant chicken IFN-α/β, IFN-γ and IL-1β in 1-day-and 3-week-old chickens. Vacccine, 18: 2147-2154.
Sekellick, M.J., A.F. Ferrandino, D.A. Hopkins and P.I. Marcus, 1994. Chicken interferon gene: Cloning, expression and analysis. J. Interferon Res., 14: 71-79.
Sick, G., U. Schultz, U. Munster, J. Meier, B. Kaspers and P. Staeheli, 1998. Promoter structures and differential responses to viral and nonviral inducers of chicken type I interferon genes. J. Biol. Chem., 273: 9749-9754.
Stone, H., B. Mitchell and M. Brugh, 1997. In ovo vaccination of chicken embryos with experimental Newcastle disease and avian influenza oil-emulsion vaccines. Avian Dis., 41: 856-863.
Ulmer, J.B., J.J. Donnelly, S.E. Parker, G.H. Rhodes and P.L. Felgner et al., 1993. Heterologous protection against influenza by injection of DNA encoding a viral protein. Science, 259: 1745-1749.
Wakenell, P.S. and J.M. Sharma, 1996. Chicken embryonal vaccination with avian infectious bronchitis virus. Am. J. Vet. Res., 47: 933-938.
Wang, L., D. Junker and E.W. Collisson, 1993. Evidence of natural recombination within the S1 gene of infectious bronchitis virus. Virology, 192: 710-716.
Wang, X., W.M. Schnitzlein, D.N. Tripathy, T. Girshick and M.I. Khan, 2001. Construction and immunogenicity studies of recombinant fowl poxvirus containing the S1 gene of massachusetts 41 strain of infectious bronchitis virus. Avian Dis., 46: 831-838.
Wang, X. and M.I. Khan, 2000. Molecular characterization of an infectious bronchitis virus strain isolated from an outbreak in vaccinated layers. Avian Dis., 44: 1000-1006.
Zhou, J.Y., J.X. Wu, L.Q. Cheng, X.J. Zheng, H. Gong, S.B. Shang and E.M. Zhou, 2003. Expression of immunogenic S1 glycoprotein of infectious bronchitis virus in transgenic potatoes. J. Virol., 77: 9090-9093.
Moura, L., M. Liu and V.N. Vakharia, 2007. Evaluation of in ovo delivery system for plasmid DNA vaccination. Int. J. Poult. Sci., 6: 776-783.
Downloads
Published
Issue
Section
License
Copyright (c) 2009 Asian Network for Scientific Information
This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
